TY - JOUR
T1 - Two-photon confocal microscopy
T2 - A nondestructive method for studying wound healing
AU - Navarro, Fernando A.
AU - So, Peter T C
AU - Nirmalan, Rubin
AU - Kropf, Nina
AU - Sakaguchi, Farrant
AU - Park, Christine S.
AU - Lee, Hoon B.
AU - Orgill, Dennis P.
PY - 2004/7
Y1 - 2004/7
N2 - Two-photon confocal microscopy is a new technology useful in nondestructive analysis of tissue. The pattern generated from laser-excited autofluorescence and second harmonic signals can be analyzed to construct a three-dimensional, microanatomical, structural image. The healing of full-thickness guinea pig skin wounds was studied over a period of 28 days using two-photon confocal microscopy. Three-dimensional data were rendered from two-dimensional images and compared with conventional, en face, histologic sections. Two-photon confocal microscopy images show resolution of muscle, fascia fibers, collagen fibers, inflammatory cells, blood vessels, and hair. Although these images do not currently have the resolution of standard histology, the ability to noninvasively acquire three-dimensional images of skin promises to be an important tool in wound-healing studies.
AB - Two-photon confocal microscopy is a new technology useful in nondestructive analysis of tissue. The pattern generated from laser-excited autofluorescence and second harmonic signals can be analyzed to construct a three-dimensional, microanatomical, structural image. The healing of full-thickness guinea pig skin wounds was studied over a period of 28 days using two-photon confocal microscopy. Three-dimensional data were rendered from two-dimensional images and compared with conventional, en face, histologic sections. Two-photon confocal microscopy images show resolution of muscle, fascia fibers, collagen fibers, inflammatory cells, blood vessels, and hair. Although these images do not currently have the resolution of standard histology, the ability to noninvasively acquire three-dimensional images of skin promises to be an important tool in wound-healing studies.
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U2 - 10.1097/01.PRS.0000128374.20913.4B
DO - 10.1097/01.PRS.0000128374.20913.4B
M3 - Article
C2 - 15220579
AN - SCOPUS:3142779336
SN - 0032-1052
VL - 114
SP - 121
EP - 128
JO - Plastic and reconstructive surgery
JF - Plastic and reconstructive surgery
IS - 1
ER -