Two Photon Confocal Microscopy in wound healing

Fernando A. Navarro, Peter T C So, Antoine Driessen, Nina Kropf, Christine S. Park, Juan C. Huertas, Hoon B. Lee, Dennis P. Orgill

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Advances in histopathology and immunohistochemestry have allowed for precise microanatomic detail of tissues. Two Photon Confocal Microscopy (TPCM) is a new technology useful in non-destructive analysis of tissue. Laser light excites the natural florophores, NAD(P)H and NADP+ and the scattering patterns of the emitted light are analyzed to reconstruct microanatomic features. Guinea pig skin was studied using TPCM and skin preparation methods including chemical depilation and tape stripping. Results of TPCM were compared with conventional hematoxylin and eosin microscopy. Two-dimensional images were rendered from the three dimensional reconstructions. Images of deeper layers including basal cells and the dermo-epidermal junction improved after removing the stratum corneum with chemical depilation or tape stripping. TCPM allows good resolution of corneocytes, basal cells and collagen fibers and shows promise as a non-destructive method to study wound healing.

Original languageEnglish (US)
Pages (from-to)27-40
Number of pages14
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - 2001

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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