TY - JOUR
T1 - Two placental hormones are agonists in stimulating megakaryocyte growth and differentiation
AU - Zhou, Beiyan
AU - Lum, Hillary E.
AU - Lin, Jiandie
AU - Linzer, Daniel I.H.
PY - 2002/11/1
Y1 - 2002/11/1
N2 - Previously, we demonstrated that a placental hormone, PRL-like protein E, stimulates megakaryocyte growth and differentiation. We now find that PRL-like protein E and a second placental hormone, PRL-like protein F (PLP-F), bind the same receptor. PLP-F, which is produced later in pregnancy, might therefore act as either an agonist or antagonist of PRL-like protein E. To resolve this question, we produced recombinant PLP-F in mammalian cell cultures, purified the secreted glycoprotein hormone, and determined its activity in primary mouse bone marrow cultures. PLP-F induces megakaryocyte differentiation and megakaryocyte progenitor growth in a dose-dependent manner, with significant activity detected at a concentration as low as 50 ng/ml. PLP-F in maternal serum reaches at least 1 μg/ml on gestational d 14.5, and thus the biological activity of PLP-F is detected at physiological concentrations. These results show that PRL-like proteins E and F have the same stimulatory effects on megakaryocyte growth and differentiation, and therefore represent gestation stage-specific agonists.
AB - Previously, we demonstrated that a placental hormone, PRL-like protein E, stimulates megakaryocyte growth and differentiation. We now find that PRL-like protein E and a second placental hormone, PRL-like protein F (PLP-F), bind the same receptor. PLP-F, which is produced later in pregnancy, might therefore act as either an agonist or antagonist of PRL-like protein E. To resolve this question, we produced recombinant PLP-F in mammalian cell cultures, purified the secreted glycoprotein hormone, and determined its activity in primary mouse bone marrow cultures. PLP-F induces megakaryocyte differentiation and megakaryocyte progenitor growth in a dose-dependent manner, with significant activity detected at a concentration as low as 50 ng/ml. PLP-F in maternal serum reaches at least 1 μg/ml on gestational d 14.5, and thus the biological activity of PLP-F is detected at physiological concentrations. These results show that PRL-like proteins E and F have the same stimulatory effects on megakaryocyte growth and differentiation, and therefore represent gestation stage-specific agonists.
UR - http://www.scopus.com/inward/record.url?scp=0036827783&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036827783&partnerID=8YFLogxK
U2 - 10.1210/en.2002-220447
DO - 10.1210/en.2002-220447
M3 - Article
C2 - 12399423
AN - SCOPUS:0036827783
VL - 143
SP - 4281
EP - 4286
JO - Endocrinology
JF - Endocrinology
SN - 0013-7227
IS - 11
ER -