Type II PtdIns 4-kinase β associates with CD4-p56lck complex and is involved in CD4 receptor signaling

Ranjeet Kumar Sinha*, Naveen Bojjireddy, Deepak Kanojia, Gosukonda Subrahmanyam

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Type II phosphatidylinositol (PtdIns) 4-kinases are involved in the synthesis of PtdIns 4-phosphates and modulate various cell functions like, intracellular signaling, cytoskeletal rearrangements, vesicular trafficking, and pathogen invasion. In CD3 receptor activated T cells, a type II PtdIns 4-kinase β is recruited to CD3 receptor zeta and plays a role in intracellular calcium release and probably in actin cytoskeleton reorganization. T cell receptor mediated activation is supported by CD4 receptor. The role of type II PtdIns 4-kinase β in CD4 receptor-mediated signaling was addressed in the present manuscript. Crosslinking of CD4 receptors with monoclonal antibodies showed an increase in CD4-associated PtdIns 4-kinase activity and requires p56lck activity. Biochemical characterization suggests that it belongs to type II PtdIns 4-kinase family. shRNA mediated knockdown of type II PtdIns 4-kinase β showed abrogation of CD4 receptor induced intracellular calcium release. These results suggest that type II PtdIns 4-kinase β plays an integral part in CD4 receptor-mediated signaling.

Original languageEnglish (US)
Pages (from-to)231-239
Number of pages9
JournalMolecular and Cellular Biochemistry
Volume395
Issue number1-2
DOIs
StatePublished - Oct 2014
Externally publishedYes

Keywords

  • CD4 receptor
  • PtdIns 4-kinases
  • T cell activation

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

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