Types of chemokines expressed by murine dendritic cell line

Jingzhu Guo, Shunyou Gong, Laiping Xie

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Objective To analyze the types of chemokines expressed in murine medullary thymic dendritic cell line (MTDC).Methods AMTDC line was established.RT-PCR was used to amplify the cDNA fragments of SDF-1, IP-10, KC, MCP-1, and lymphotactin expressed by MTDC. Nucleotide sequencing and dot blotting analysis were performed to confirm their existence.Results Five cDNA fragments SDF-1, KC, IP-10, MCP-1, and lymphotactin, were amplified from MTDC. The existence of SDF-1, KC, and lymphotactin was confirmed by nucleotide wequencing, and that of IP-10, MCP-1, and lymphotactin was confirmed by dot blotting.The supernatant of MTDC exhibited chemoattractant activities to CD4 - ∼ CD8 - thymocytes with a chemotactic index (CI)of 3.4.Conclusion There is a lot of chemokines in MTDC, including C-X-C subfamily (SDF-1, KC, and IP-10)CC subfamily (MCP-1), and C subfamily (lymphotactin).The suparnatant of MTDC has moderately chemotactic activities to CD4 -∼ CD8 - thymocytes, consistent with the biological function of SDF-1.

Original languageEnglish (US)
Pages (from-to)93-96
Number of pages4
JournalNational Medical Journal of China
Volume81
Issue number2
StatePublished - Jan 1 2001

Keywords

  • Chemical analysis
  • Chemokines
  • Thymic dendritic cells

ASJC Scopus subject areas

  • Medicine(all)

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