Types of chemokines expressed by murine dendritic cell line

Objective To analyze the types of chemokines expressed in murine medullary thymic dendritic cell line (MTDC).Methods AMTDC line was established.RT-PCR was used to amplify the cDNA fragments of SDF-1, IP-10, KC, MCP-1, and lymphotactin expressed by MTDC. Nucleotide sequencing and dot blotting analysis were performed to confirm their existence.Results Five cDNA fragments SDF-1, KC, IP-10, MCP-1, and lymphotactin, were amplified from MTDC. The existence of SDF-1, KC, and lymphotactin was confirmed by nucleotide wequencing, and that of IP-10, MCP-1, and lymphotactin was confirmed by dot blotting.The supernatant of MTDC exhibited chemoattractant activities to CD₄^- ∼ CD₈^- thymocytes with a chemotactic index (CI)of 3.4.Conclusion There is a lot of chemokines in MTDC, including C-X-C subfamily (SDF-1, KC, and IP-10)CC subfamily (MCP-1), and C subfamily (lymphotactin).The suparnatant of MTDC has moderately chemotactic activities to CD₄^-∼ CD₈^- thymocytes, consistent with the biological function of SDF-1.