U1 and U2 small nuclear RNAs are present in nuclear speckles

Sui Huang; David L. Spector

doi:10.1073/pnas.89.1.305

U1 and U2 small nuclear RNAs are present in nuclear speckles

Sui Huang, David L. Spector^*

^*Corresponding author for this work

Cell & Developmental Biology

Research output: Contribution to journal › Article › peer-review

114 Scopus citations

Abstract

The localization of UI and U2 small nuclear RNAs (snRNAs) has been examined by in situ hybridization using 2′-O-alkyl oligonucleotide probes. We have found that these snRNAs, which are essential for pre-mRNA splicing, localize in a speckled distribution, in addition to being present in three or four foci, in HeLa cell nuclei. However, in cells of defined passage, such as Detroit 551 and WI-38 fibroblasts, these snRNAs are concentrated in nuclear speckles, and foci are not observed. The speckled distribution of U1 and 112 snRNAs is coincident with the speckled regions enriched in small nuclear ribonucleoprotein particle (snRNP) proteins and the essential non-snRNP splicing factor SC-35. The localization of these key components of the pre-mRN A splicing machinery to speckled nuclear regions suggests that these regions may be involved in pre-mRNA splicing.

Original language	English (US)
Pages (from-to)	305-308
Number of pages	4
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	89
Issue number	1
DOIs	https://doi.org/10.1073/pnas.89.1.305
State	Published - 1992

Keywords

Nuclear organization
RNA processing

ASJC Scopus subject areas

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AU - Spector, David L.

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AB - The localization of UI and U2 small nuclear RNAs (snRNAs) has been examined by in situ hybridization using 2′-O-alkyl oligonucleotide probes. We have found that these snRNAs, which are essential for pre-mRNA splicing, localize in a speckled distribution, in addition to being present in three or four foci, in HeLa cell nuclei. However, in cells of defined passage, such as Detroit 551 and WI-38 fibroblasts, these snRNAs are concentrated in nuclear speckles, and foci are not observed. The speckled distribution of U1 and 112 snRNAs is coincident with the speckled regions enriched in small nuclear ribonucleoprotein particle (snRNP) proteins and the essential non-snRNP splicing factor SC-35. The localization of these key components of the pre-mRN A splicing machinery to speckled nuclear regions suggests that these regions may be involved in pre-mRNA splicing.

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U1 and U2 small nuclear RNAs are present in nuclear speckles

Abstract

Keywords

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