Abstract
Rare leukemic B cells presented in therapeutic T cell populations can cause disease relapse due to their ability to escape from the recognition of T cells. Unfortunately, the traditional cell purification method, such as FACS, is not sensitive enough for rare cell analysis. Here, we implemented our microfluidics-mediated immunomagnetic sorting approach, named magnetic ranking cytometry (MagRC), to enable efficient detection of rare B cells in exceeding T cell background. A quantitative comparison revealed that our MagRC approach outperformed the FACS and achieved a single-cell resolution. We believe the MagRC approach is promising for assessing and improving the safeness of immunotherapy.
| Original language | English (US) |
|---|---|
| Title of host publication | MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 729-730 |
| Number of pages | 2 |
| ISBN (Electronic) | 9781733419017 |
| State | Published - 2020 |
| Event | 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 - Virtual, Online Duration: Oct 4 2020 → Oct 9 2020 |
Publication series
| Name | MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
|---|
Conference
| Conference | 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 |
|---|---|
| City | Virtual, Online |
| Period | 10/4/20 → 10/9/20 |
Funding
This research was supported in part by the Canadian Institutes of Health Research (grant no. FDN-148415) and the Collaborative Health Research Projects program (CIHR/NSERC partnered).
Keywords
- Cell Sorting
- Cell Therapy
- Immunotherapy
- Rare Cell
ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Bioengineering
- General Chemistry
- Control and Systems Engineering