Ultrasensitive detection and depletion of rare leukemic b cells in t cell populations via microfluidics-mediated immunomagnetic cell ranking

Zongjie Wang, Shana O. Kelley*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Rare leukemic B cells presented in therapeutic T cell populations can cause disease relapse due to their ability to escape from the recognition of T cells. Unfortunately, the traditional cell purification method, such as FACS, is not sensitive enough for rare cell analysis. Here, we implemented our microfluidics-mediated immunomagnetic sorting approach, named magnetic ranking cytometry (MagRC), to enable efficient detection of rare B cells in exceeding T cell background. A quantitative comparison revealed that our MagRC approach outperformed the FACS and achieved a single-cell resolution. We believe the MagRC approach is promising for assessing and improving the safeness of immunotherapy.

Original languageEnglish (US)
Title of host publicationMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages729-730
Number of pages2
ISBN (Electronic)9781733419017
StatePublished - 2020
Event24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 - Virtual, Online
Duration: Oct 4 2020Oct 9 2020

Publication series

NameMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
CityVirtual, Online
Period10/4/2010/9/20

Keywords

  • Cell Sorting
  • Cell Therapy
  • Immunotherapy
  • Rare Cell

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering
  • General Chemistry
  • Control and Systems Engineering

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