Ultrastructural alterations in the aqueous outflow pathway of adult buphthalmic rabbits

Paul A. Knepper; David G. McLone; William Goossens; Terry Vanden Hoek; Russell G. Higbee

doi:10.1016/0014-4835(91)90053-H

Ultrastructural alterations in the aqueous outflow pathway of adult buphthalmic rabbits

Paul A. Knepper^*, David G. McLone, William Goossens, Terry Vanden Hoek, Russell G. Higbee

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The aqueous outflow pathway of adult rabbit eyes with congenital glaucoma (buphthalmos) was examined by light microscopy and by scanning and transmission electron microscopy. The morphology of the buphthalmic rabbit aqueous outflow pathway was markedly abnormal when examined at 6 months, 1 yr, and 2 yr displaying apparent loss and/or compression of the iris pillars, dilation of the intertrabecular spaces, loss of endothelial cell-to-cell association and disorganization of trabecular lamellae, and posterior displacement of the aqueous plexus. In addition, the trabecular meshwork lamellae were observed only adjacent to the sclera and the inner portion of the trabecular meshwork was limited to swirls of collagen with scattered cells. These morphological findings suggest that the disease process in the rabbit principally involves an alteration in the differentiation and maintenance of the structural integrity of the trabecular meshwork. The loss of structural support of the buphthalmic trabecular meshwork may be a factor in the wide variation in intraocular pressure and may allow for compression of the trabecular meshwork against the aqueous plexus.

Original language	English (US)
Pages (from-to)	525-533
Number of pages	9
Journal	Experimental eye research
Volume	52
Issue number	5
DOIs	https://doi.org/10.1016/0014-4835(91)90053-H
State	Published - May 1991

Keywords

buphthalmos
congenital glaucoma
intraocular pressure
rabbit
trabecular meshwork

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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10.1016/0014-4835(91)90053-H

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title = "Ultrastructural alterations in the aqueous outflow pathway of adult buphthalmic rabbits",

abstract = "The aqueous outflow pathway of adult rabbit eyes with congenital glaucoma (buphthalmos) was examined by light microscopy and by scanning and transmission electron microscopy. The morphology of the buphthalmic rabbit aqueous outflow pathway was markedly abnormal when examined at 6 months, 1 yr, and 2 yr displaying apparent loss and/or compression of the iris pillars, dilation of the intertrabecular spaces, loss of endothelial cell-to-cell association and disorganization of trabecular lamellae, and posterior displacement of the aqueous plexus. In addition, the trabecular meshwork lamellae were observed only adjacent to the sclera and the inner portion of the trabecular meshwork was limited to swirls of collagen with scattered cells. These morphological findings suggest that the disease process in the rabbit principally involves an alteration in the differentiation and maintenance of the structural integrity of the trabecular meshwork. The loss of structural support of the buphthalmic trabecular meshwork may be a factor in the wide variation in intraocular pressure and may allow for compression of the trabecular meshwork against the aqueous plexus.",

keywords = "buphthalmos, congenital glaucoma, intraocular pressure, rabbit, trabecular meshwork",

author = "Knepper, {Paul A.} and McLone, {David G.} and William Goossens and {Vanden Hoek}, Terry and Higbee, {Russell G.}",

note = "Funding Information: The authors wish to thank the Crawford family for their assistance in this study, Carol Fabian for preparation of the manuscript, and Dolores Henning for editorial assistance. This study was supported in part by Grant no. EY 06662 from the National Eye Institute, the National Children{\textquoteright}s Eye Care Foundation, and the Olga Keith Wiess Scholar Award by Research to Prevent Blindness Inc. (P.A.K.).",

year = "1991",

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doi = "10.1016/0014-4835(91)90053-H",

language = "English (US)",

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pages = "525--533",

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T1 - Ultrastructural alterations in the aqueous outflow pathway of adult buphthalmic rabbits

AU - Knepper, Paul A.

AU - McLone, David G.

AU - Goossens, William

AU - Vanden Hoek, Terry

AU - Higbee, Russell G.

N1 - Funding Information: The authors wish to thank the Crawford family for their assistance in this study, Carol Fabian for preparation of the manuscript, and Dolores Henning for editorial assistance. This study was supported in part by Grant no. EY 06662 from the National Eye Institute, the National Children’s Eye Care Foundation, and the Olga Keith Wiess Scholar Award by Research to Prevent Blindness Inc. (P.A.K.).

PY - 1991/5

Y1 - 1991/5

N2 - The aqueous outflow pathway of adult rabbit eyes with congenital glaucoma (buphthalmos) was examined by light microscopy and by scanning and transmission electron microscopy. The morphology of the buphthalmic rabbit aqueous outflow pathway was markedly abnormal when examined at 6 months, 1 yr, and 2 yr displaying apparent loss and/or compression of the iris pillars, dilation of the intertrabecular spaces, loss of endothelial cell-to-cell association and disorganization of trabecular lamellae, and posterior displacement of the aqueous plexus. In addition, the trabecular meshwork lamellae were observed only adjacent to the sclera and the inner portion of the trabecular meshwork was limited to swirls of collagen with scattered cells. These morphological findings suggest that the disease process in the rabbit principally involves an alteration in the differentiation and maintenance of the structural integrity of the trabecular meshwork. The loss of structural support of the buphthalmic trabecular meshwork may be a factor in the wide variation in intraocular pressure and may allow for compression of the trabecular meshwork against the aqueous plexus.

AB - The aqueous outflow pathway of adult rabbit eyes with congenital glaucoma (buphthalmos) was examined by light microscopy and by scanning and transmission electron microscopy. The morphology of the buphthalmic rabbit aqueous outflow pathway was markedly abnormal when examined at 6 months, 1 yr, and 2 yr displaying apparent loss and/or compression of the iris pillars, dilation of the intertrabecular spaces, loss of endothelial cell-to-cell association and disorganization of trabecular lamellae, and posterior displacement of the aqueous plexus. In addition, the trabecular meshwork lamellae were observed only adjacent to the sclera and the inner portion of the trabecular meshwork was limited to swirls of collagen with scattered cells. These morphological findings suggest that the disease process in the rabbit principally involves an alteration in the differentiation and maintenance of the structural integrity of the trabecular meshwork. The loss of structural support of the buphthalmic trabecular meshwork may be a factor in the wide variation in intraocular pressure and may allow for compression of the trabecular meshwork against the aqueous plexus.

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KW - trabecular meshwork

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Ultrastructural alterations in the aqueous outflow pathway of adult buphthalmic rabbits

Abstract

Keywords

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