TY - JOUR
T1 - Ultrastructure of Ristocetin Aggregated Normal Human Platelets
T2 - Tortuous Boundaries and Swollen Granules
AU - Ts'ao, Chung‐Hsin ‐H
AU - Green, David
AU - Rossi, Ennio C.
PY - 1973/10
Y1 - 1973/10
N2 - The effects of ristocetin on human platelets were investigated by aggregometry and electron microscopy. We confirmed the finding that ristocetin induces platelet aggregation. Biphasic platelet aggregation, such as is observed with ADP, was also seen with ristocetin. The gross appearance of ristocetin aggregated specimens differed markedly from those induced by ADP or collagen. On electron microscopic examination the boundaries of the aggregated platelets were noted to be extremely tortuous with electron dense lines between membranes, and there were many swollen α‐granules. At the periphery of the aggregates, the platelets were markedly degranulated. The plasma membranes of degranulated platelets were occasionally lysed. Morphological alterations of platelets exposed to ristocetin appeared to continue even after maximum aggregation, as recorded in the aggregometer, had been achieved.
AB - The effects of ristocetin on human platelets were investigated by aggregometry and electron microscopy. We confirmed the finding that ristocetin induces platelet aggregation. Biphasic platelet aggregation, such as is observed with ADP, was also seen with ristocetin. The gross appearance of ristocetin aggregated specimens differed markedly from those induced by ADP or collagen. On electron microscopic examination the boundaries of the aggregated platelets were noted to be extremely tortuous with electron dense lines between membranes, and there were many swollen α‐granules. At the periphery of the aggregates, the platelets were markedly degranulated. The plasma membranes of degranulated platelets were occasionally lysed. Morphological alterations of platelets exposed to ristocetin appeared to continue even after maximum aggregation, as recorded in the aggregometer, had been achieved.
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U2 - 10.1111/j.1600-0609.1973.tb00131.x
DO - 10.1111/j.1600-0609.1973.tb00131.x
M3 - Article
C2 - 4777829
AN - SCOPUS:0015906459
VL - 11
SP - 287
EP - 297
JO - European Journal of Haematology
JF - European Journal of Haematology
SN - 0902-4441
IS - 4
ER -