Ultraviolet-B irradiation of leukapheresis products: Dose-response relationship with the mixed lymphocyte reaction

Ultraviolet-B (UVB) irradiation of blood constituents intensifies their anti-rejection effect in pretransplant donor-specific transfusions. UVB- induced inhibition of the mixed lymphocyte reaction (MLR) between UVB- irradiated donor cells and prospective recipient cells is a predicator of this anti-rejection effect. In order to define the dose-response relationship between the incident UVB irradiation on leukocyte concentrates and subsequent inhibition of their MLR responses, we collected 4 ± 2 x 10⁹ leukocytes (93 ± 7% lymphocytes) in 200 ml plasma from each of three volunteers by leukapheresis and exposed them to rapid, serial doses of UVB irradiation which was delivered by a blood product irradiator (4R4440 UVB Irradiator, Baxter, Inc) with aliquots removed between doses. Lymphocytes front each aliquot were placed in MLR with panel donors and studied in three groups: 1) the panel donor cells were γ-irradiated (1,500 rads) (i.e., only the UVB-irradiated cells could proliferate), 2) the UVB-irradiated cells were γ-irradiated (i.e., only the panel lymphocytes could proliferate), and 3) no γ-irradiation (i.e., both cell populations could proliferate). Each group had a similar UVB dose-related diminution in the MLR (p = .79, ANOVA). A single dose of 6 J/cm² extinguished the MLR to baseline in all groups. This dose should theoretically prevent transfused cells from producing either graft-versus host disease or allosensitization, and might heighten their tolerogenic effect. This dose will be employed in our study of donor- specific leukocyte transfusion in clinical renal transplantation.