Ultraviolet-B irradiation of leukapheresis products: Dose-response relationship with the mixed lymphocyte reaction

Preston F. Foster*, Howard Gebel, Bruce C. McLeod, Nancy Ebert, Stephen Jensik, Howard N. Sankary, Anat Tambur, James W. Williams

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Ultraviolet-B (UVB) irradiation of blood constituents intensifies their anti-rejection effect in pretransplant donor-specific transfusions. UVB- induced inhibition of the mixed lymphocyte reaction (MLR) between UVB- irradiated donor cells and prospective recipient cells is a predicator of this anti-rejection effect. In order to define the dose-response relationship between the incident UVB irradiation on leukocyte concentrates and subsequent inhibition of their MLR responses, we collected 4 ± 2 x 109 leukocytes (93 ± 7% lymphocytes) in 200 ml plasma from each of three volunteers by leukapheresis and exposed them to rapid, serial doses of UVB irradiation which was delivered by a blood product irradiator (4R4440 UVB Irradiator, Baxter, Inc) with aliquots removed between doses. Lymphocytes front each aliquot were placed in MLR with panel donors and studied in three groups: 1) the panel donor cells were γ-irradiated (1,500 rads) (i.e., only the UVB-irradiated cells could proliferate), 2) the UVB-irradiated cells were γ-irradiated (i.e., only the panel lymphocytes could proliferate), and 3) no γ-irradiation (i.e., both cell populations could proliferate). Each group had a similar UVB dose-related diminution in the MLR (p = .79, ANOVA). A single dose of 6 J/cm2 extinguished the MLR to baseline in all groups. This dose should theoretically prevent transfused cells from producing either graft-versus host disease or allosensitization, and might heighten their tolerogenic effect. This dose will be employed in our study of donor- specific leukocyte transfusion in clinical renal transplantation.

Original languageEnglish (US)
Pages (from-to)55-60
Number of pages6
JournalJournal of clinical apheresis
Volume11
Issue number2
DOIs
StatePublished - Sep 11 1996

Keywords

  • irradiation
  • leukapheresis
  • lymphocytes
  • ultraviolet

ASJC Scopus subject areas

  • Hematology

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