Unaltered anionic sites of glomerular basement membrane in aminonucleoside nephrosis

Y. S. Kanwar; M. L. Jakubowski

doi:10.1038/ki.1984.65

Unaltered anionic sites of glomerular basement membrane in aminonucleoside nephrosis

Y. S. Kanwar, M. L. Jakubowski

Pathology

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

Quantitative electron microscopic autoradiography was employed to determine the changes in the binding of ¹²⁵I-cationic ferritin (CFI, pI ~ 7.2 to 7.4) to the anionic sites of the glomerular basement membrane (GBM) of rats following the induction of nephrosis. Animals were rendered nephrotic by a single intravenous injection of puromycin aminonucleoside (PAN) and sacrificed 0, 7, 14, and 21 days after its administration. CFI (10 mg/mCi/100 g body weight) was given intravenously. The kidneys were subsequently fixed by perfusion and processed for electron microscopic autoradiography. The mean grain densities over the GBMs on 0, 7, 14, and 21 days of PAN nephrosis were 1.50 ± 0.04, 1.49 ± 0.05, 1.50 ± 0.05, and 1.51 ± 0.04, respectively. These results indicate that there are no significant alterations in the anionic sites rich in heparan sulfate proteoglycan during the entire course of PAN nephrosis.

Original language	English (US)
Pages (from-to)	613-618
Number of pages	6
Journal	Kidney international
Volume	25
Issue number	4
DOIs	https://doi.org/10.1038/ki.1984.65
State	Published - 1984

Funding

This research was supported by National Institutes of Health grant AM28492. Dr. Y. S. Kanwar is a recipient of a Research Career Development Award (AMOIO18) from the National Institutes of Health. The authors acknowledge suggestions made by Dr. L. J. Rosenzweig during the course of this investigation.

ASJC Scopus subject areas

Nephrology

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10.1038/ki.1984.65

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title = "Unaltered anionic sites of glomerular basement membrane in aminonucleoside nephrosis",

abstract = "Quantitative electron microscopic autoradiography was employed to determine the changes in the binding of 125I-cationic ferritin (CFI, pI ~ 7.2 to 7.4) to the anionic sites of the glomerular basement membrane (GBM) of rats following the induction of nephrosis. Animals were rendered nephrotic by a single intravenous injection of puromycin aminonucleoside (PAN) and sacrificed 0, 7, 14, and 21 days after its administration. CFI (10 mg/mCi/100 g body weight) was given intravenously. The kidneys were subsequently fixed by perfusion and processed for electron microscopic autoradiography. The mean grain densities over the GBMs on 0, 7, 14, and 21 days of PAN nephrosis were 1.50 ± 0.04, 1.49 ± 0.05, 1.50 ± 0.05, and 1.51 ± 0.04, respectively. These results indicate that there are no significant alterations in the anionic sites rich in heparan sulfate proteoglycan during the entire course of PAN nephrosis.",

author = "Kanwar, {Y. S.} and Jakubowski, {M. L.}",

note = "Funding Information: This research was supported by National Institutes of Health grant AM28492. Dr. Y. S. Kanwar is a recipient of a Research Career Development Award (AMOIO18) from the National Institutes of Health. The authors acknowledge suggestions made by Dr. L. J. Rosenzweig during the course of this investigation.",

year = "1984",

doi = "10.1038/ki.1984.65",

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AU - Kanwar, Y. S.

AU - Jakubowski, M. L.

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PY - 1984

Y1 - 1984

N2 - Quantitative electron microscopic autoradiography was employed to determine the changes in the binding of 125I-cationic ferritin (CFI, pI ~ 7.2 to 7.4) to the anionic sites of the glomerular basement membrane (GBM) of rats following the induction of nephrosis. Animals were rendered nephrotic by a single intravenous injection of puromycin aminonucleoside (PAN) and sacrificed 0, 7, 14, and 21 days after its administration. CFI (10 mg/mCi/100 g body weight) was given intravenously. The kidneys were subsequently fixed by perfusion and processed for electron microscopic autoradiography. The mean grain densities over the GBMs on 0, 7, 14, and 21 days of PAN nephrosis were 1.50 ± 0.04, 1.49 ± 0.05, 1.50 ± 0.05, and 1.51 ± 0.04, respectively. These results indicate that there are no significant alterations in the anionic sites rich in heparan sulfate proteoglycan during the entire course of PAN nephrosis.

AB - Quantitative electron microscopic autoradiography was employed to determine the changes in the binding of 125I-cationic ferritin (CFI, pI ~ 7.2 to 7.4) to the anionic sites of the glomerular basement membrane (GBM) of rats following the induction of nephrosis. Animals were rendered nephrotic by a single intravenous injection of puromycin aminonucleoside (PAN) and sacrificed 0, 7, 14, and 21 days after its administration. CFI (10 mg/mCi/100 g body weight) was given intravenously. The kidneys were subsequently fixed by perfusion and processed for electron microscopic autoradiography. The mean grain densities over the GBMs on 0, 7, 14, and 21 days of PAN nephrosis were 1.50 ± 0.04, 1.49 ± 0.05, 1.50 ± 0.05, and 1.51 ± 0.04, respectively. These results indicate that there are no significant alterations in the anionic sites rich in heparan sulfate proteoglycan during the entire course of PAN nephrosis.

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Unaltered anionic sites of glomerular basement membrane in aminonucleoside nephrosis

Abstract

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