Uncoupling neurogenic gene networks in the drosophila embryo

William A. Rogers; Yogesh Goyal; Kei Yamaya; Stanislav Y. Shvartsman; Michael S. Levine

doi:10.1101/gad.297150.117

Uncoupling neurogenic gene networks in the drosophila embryo

William A. Rogers, Yogesh Goyal, Kei Yamaya, Stanislav Y. Shvartsman, Michael S. Levine^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

The EGF signaling pathway specifies neuronal identities in the Drosophila embryo by regulating developmental patterning genes such as intermediate neuroblasts defective (ind).EGFR is activated in the ventral midline and neurogenic ectoderm by the Spitz ligand,which is processed by the Rhomboid protease. CRISPR/Cas9 was used to delete defined rhomboid enhancers mediating expression at each site of Spitz processing. Surprisingly, the neurogenic ectoderm, not the ventral midline, was found to be the dominant source of EGF patterning activity. We suggest that Drosophila is undergoing an evolutionary transition in central nervous system (CNS)-organizing activity from the ventral midline to the neurogenic ectoderm.

Original language	English (US)
Pages (from-to)	634-638
Number of pages	5
Journal	Genes and Development
Volume	31
Issue number	7
DOIs	https://doi.org/10.1101/gad.297150.117
State	Published - Apr 1 2017
Externally published	Yes

Keywords

Central nervous system
Drosophila embryo
EGFR regulatory networks
Intermediate neuroblasts defective
Rhomboid

ASJC Scopus subject areas

Genetics
Developmental Biology

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10.1101/gad.297150.117

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title = "Uncoupling neurogenic gene networks in the drosophila embryo",

abstract = "The EGF signaling pathway specifies neuronal identities in the Drosophila embryo by regulating developmental patterning genes such as intermediate neuroblasts defective (ind).EGFR is activated in the ventral midline and neurogenic ectoderm by the Spitz ligand,which is processed by the Rhomboid protease. CRISPR/Cas9 was used to delete defined rhomboid enhancers mediating expression at each site of Spitz processing. Surprisingly, the neurogenic ectoderm, not the ventral midline, was found to be the dominant source of EGF patterning activity. We suggest that Drosophila is undergoing an evolutionary transition in central nervous system (CNS)-organizing activity from the ventral midline to the neurogenic ectoderm.",

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note = "Funding Information: We thank members of the Levine and Shvartsman laboratories for their invaluable comments and advice. We also thank Hongtao Chen and Thomas Gregor for providing many of the CRISPR/Cas9 plasmids used in our experiments, and Gary Laevsky and Evangelos G. Gatzogiannis for their expert help with imaging. W.A.R. was supported by a National Institutes of Health (NIH) grant to M.S.L. (R35 GM118147). Y.G. and K.Y. were supported by an NIH grant to S.Y.S. (R01 GM086537). W.A.R., Y.G., S.Y.S., and M.S.L. conceived and designed the project; W.A.R. designed the CRISPR/Cas9 mutant flies; Y.G.,W.A.R., and K.Y. performed the imaging experiments;W.A.R. and Y.G. analyzed the results; andW.A.R., Y.G., S.Y. S., and M.S.L. wrote the manuscript. Publisher Copyright: {\textcopyright} 2017 Rogers et al.",

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Uncoupling neurogenic gene networks in the drosophila embryo

Abstract

Keywords

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