Unexpected role for MHC II-peptide complexes in shaping CD8 T-cell expansion and differentiation in vivo

Jeong Su Do, Anna Valujskikh, Dario A.A. Vignali, Robert L. Fairchild, Booki Min*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Here we report a unique role for MHC II-peptide complexes incontrolling immune responses of naïve CD8 T cells. Compared with CD8 T cells from WT mice, CD8 T cells isolated from MHC II-/- mice hyperproliferated under lymphopenic conditions, differentiated into effector cells producing proinflammatory cytokines, and mediated more severe tissue inflammation. The elevated responses of MHC II-/- CD8 T cells were due to the absence of MHC II, but not CD4, T cells. The hyperreactivity appeared to be a feature of mature T cells, given its absence in CD8 single positive thymocytesderived from MHC II-/- mice. Expression of the MHC II ligand LAG3 was markedly enhanced during in vivo activation of MHC II-/-CD8 T cells, and blockade of MHC II-LAG3 interactions furtherenhanced T-cell expansion. Importantly, CD8 T cells isolated from H-2M-/- mice expressing WT levels of MHC II also displayed hyperresponsiveness similar to that of MHC II-/- CD8 T cells, suggestingthat peptides presented on MHC II are involved in the control ofCD8 T-cell responses. Our results uncover a previously undefinedMHC II-dependent regulation that tunes CD8 T-cell reactivity and may have implications for an improved understanding of CD8 T-cell homeostasis and functions.

Original languageEnglish (US)
Pages (from-to)12698-12703
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number31
DOIs
StatePublished - Jul 31 2012
Externally publishedYes

Keywords

  • Lymphocytes
  • Lymphopenia
  • Proliferation
  • Self-peptide

ASJC Scopus subject areas

  • General

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