Here we report a unique role for MHC II-peptide complexes incontrolling immune responses of naïve CD8 T cells. Compared with CD8 T cells from WT mice, CD8 T cells isolated from MHC II-/- mice hyperproliferated under lymphopenic conditions, differentiated into effector cells producing proinflammatory cytokines, and mediated more severe tissue inflammation. The elevated responses of MHC II-/- CD8 T cells were due to the absence of MHC II, but not CD4, T cells. The hyperreactivity appeared to be a feature of mature T cells, given its absence in CD8 single positive thymocytesderived from MHC II-/- mice. Expression of the MHC II ligand LAG3 was markedly enhanced during in vivo activation of MHC II-/-CD8 T cells, and blockade of MHC II-LAG3 interactions furtherenhanced T-cell expansion. Importantly, CD8 T cells isolated from H-2M-/- mice expressing WT levels of MHC II also displayed hyperresponsiveness similar to that of MHC II-/- CD8 T cells, suggestingthat peptides presented on MHC II are involved in the control ofCD8 T-cell responses. Our results uncover a previously undefinedMHC II-dependent regulation that tunes CD8 T-cell reactivity and may have implications for an improved understanding of CD8 T-cell homeostasis and functions.
|Original language||English (US)|
|Number of pages||6|
|Journal||Proceedings of the National Academy of Sciences of the United States of America|
|State||Published - Jul 31 2012|
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