Uniform polarity microtubule assemblies imaged in native brain tissue by second-harmonic generation microscopy

Daniel A. Dombeck; Karl A. Kasischke; Harshad D. Vishwasrao; Martin Ingelsson; Bradley T. Hyman; Watt W. Webb

doi:10.1073/pnas.0731953100

Uniform polarity microtubule assemblies imaged in native brain tissue by second-harmonic generation microscopy

Daniel A. Dombeck, Karl A. Kasischke, Harshad D. Vishwasrao, Martin Ingelsson, Bradley T. Hyman, Watt W. Webb^*

^*Corresponding author for this work

Neurobiology

Research output: Contribution to journal › Article › peer-review

262 Scopus citations

Abstract

Microtubule (MT) ensemble polarity is a diagnostic determinant of the structure and function of neuronal processes. Here, polarized MT structures are selectively imaged with second-harmonic generation (SHG) microscopy in native brain tissue. This SHG is found to colocalize with axons in both brain slices and cultured neurons. Because SHG arises only from noninversion symmetric structures, the uniform polarity of axonal MTs leads to the observed signal, whereas the mixed polarity in dendrites leads to destructive interference. SHG imaging provides a tool to investigate the kinetics and function of MT ensemble polarity in dynamic native brain tissue structures and other subcellular motility structures based on polarized MTs.

Original language	English (US)
Pages (from-to)	7081-7086
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	100
Issue number	12
DOIs	https://doi.org/10.1073/pnas.0731953100
State	Published - Jun 10 2003

Funding

ASJC Scopus subject areas

General

Access to Document

10.1073/pnas.0731953100

Cite this

@article{e6ecb01366614ad2b053118c80d15fad,

title = "Uniform polarity microtubule assemblies imaged in native brain tissue by second-harmonic generation microscopy",

abstract = "Microtubule (MT) ensemble polarity is a diagnostic determinant of the structure and function of neuronal processes. Here, polarized MT structures are selectively imaged with second-harmonic generation (SHG) microscopy in native brain tissue. This SHG is found to colocalize with axons in both brain slices and cultured neurons. Because SHG arises only from noninversion symmetric structures, the uniform polarity of axonal MTs leads to the observed signal, whereas the mixed polarity in dendrites leads to destructive interference. SHG imaging provides a tool to investigate the kinetics and function of MT ensemble polarity in dynamic native brain tissue structures and other subcellular motility structures based on polarized MTs.",

author = "Dombeck, {Daniel A.} and Kasischke, {Karl A.} and Vishwasrao, {Harshad D.} and Martin Ingelsson and Hyman, {Bradley T.} and Webb, {Watt W.}",

year = "2003",

month = jun,

day = "10",

doi = "10.1073/pnas.0731953100",

language = "English (US)",

volume = "100",

pages = "7081--7086",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "12",

}

Uniform polarity microtubule assemblies imaged in native brain tissue by second-harmonic generation microscopy. / Dombeck, Daniel A.; Kasischke, Karl A.; Vishwasrao, Harshad D. et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 12, 10.06.2003, p. 7081-7086.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Uniform polarity microtubule assemblies imaged in native brain tissue by second-harmonic generation microscopy

AU - Dombeck, Daniel A.

AU - Kasischke, Karl A.

AU - Vishwasrao, Harshad D.

AU - Ingelsson, Martin

AU - Hyman, Bradley T.

AU - Webb, Watt W.

PY - 2003/6/10

Y1 - 2003/6/10

N2 - Microtubule (MT) ensemble polarity is a diagnostic determinant of the structure and function of neuronal processes. Here, polarized MT structures are selectively imaged with second-harmonic generation (SHG) microscopy in native brain tissue. This SHG is found to colocalize with axons in both brain slices and cultured neurons. Because SHG arises only from noninversion symmetric structures, the uniform polarity of axonal MTs leads to the observed signal, whereas the mixed polarity in dendrites leads to destructive interference. SHG imaging provides a tool to investigate the kinetics and function of MT ensemble polarity in dynamic native brain tissue structures and other subcellular motility structures based on polarized MTs.

AB - Microtubule (MT) ensemble polarity is a diagnostic determinant of the structure and function of neuronal processes. Here, polarized MT structures are selectively imaged with second-harmonic generation (SHG) microscopy in native brain tissue. This SHG is found to colocalize with axons in both brain slices and cultured neurons. Because SHG arises only from noninversion symmetric structures, the uniform polarity of axonal MTs leads to the observed signal, whereas the mixed polarity in dendrites leads to destructive interference. SHG imaging provides a tool to investigate the kinetics and function of MT ensemble polarity in dynamic native brain tissue structures and other subcellular motility structures based on polarized MTs.

UR - http://www.scopus.com/inward/record.url?scp=0038472234&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038472234&partnerID=8YFLogxK

U2 - 10.1073/pnas.0731953100

DO - 10.1073/pnas.0731953100

M3 - Article

C2 - 12766225

AN - SCOPUS:0038472234

SN - 0027-8424

VL - 100

SP - 7081

EP - 7086

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 12

ER -

Uniform polarity microtubule assemblies imaged in native brain tissue by second-harmonic generation microscopy

Abstract

Funding

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this