Unit resolution mass spectra of 112 kDa molecules with 3 Da accuracy

Neil L. Kelleher; Michael W. Senko; Marshall M. Siegel; Fred W. McLafferty

doi:10.1016/S1044-0305(97)00060-3

Unit resolution mass spectra of 112 kDa molecules with 3 Da accuracy

Neil L. Kelleher, Michael W. Senko, Marshall M. Siegel, Fred W. McLafferty^*

^*Corresponding author for this work

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

123 Scopus citations

Abstract

Previously, the highest molecular weight for a sample yielding a unit resolution mass spectrum was 67 kDa (marginal at 86 kDa), obtained with a 6.2 T Fourier-transform mass spectrometer with electrospray ionization. Now with a 9.4 T instrument, resolving power of 170,000 has been achieved for chondroitinase I (997 amino acids) and II (990 amino acids), making possible molecular weight assignments of 112,509 and 111,714, respectively, versus 112,508 and 111,713 calculated. Assisting these assignments was the noise reduction in the resolved isotopic peaks achieved by the time domain data sampling technique introduced by Senko, Marshall, and co-workers.

Original language	English (US)
Pages (from-to)	380-383
Number of pages	4
Journal	Journal of the American Society for Mass Spectrometry
Volume	8
Issue number	4
DOIs	https://doi.org/10.1016/S1044-0305(97)00060-3
State	Published - Apr 1997

Funding

The authors thank Kiran Khandke fchondroitinase samples), Alan Marshall (National High-Field FT-ICR MS Facility, NHMFL, NSF CHE-94-130081, the National Institutes of Health (FWM, GM16609; NLK, Cell & Molecular Biology Training Grant No. 08-T2GM07273), and the American Chemical Society (NLK, Division of Analytical Chemistry full fellowship sponsored by Perkin-Elmer Corporation).

ASJC Scopus subject areas

Structural Biology
Spectroscopy

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10.1016/S1044-0305(97)00060-3

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title = "Unit resolution mass spectra of 112 kDa molecules with 3 Da accuracy",

abstract = "Previously, the highest molecular weight for a sample yielding a unit resolution mass spectrum was 67 kDa (marginal at 86 kDa), obtained with a 6.2 T Fourier-transform mass spectrometer with electrospray ionization. Now with a 9.4 T instrument, resolving power of 170,000 has been achieved for chondroitinase I (997 amino acids) and II (990 amino acids), making possible molecular weight assignments of 112,509 and 111,714, respectively, versus 112,508 and 111,713 calculated. Assisting these assignments was the noise reduction in the resolved isotopic peaks achieved by the time domain data sampling technique introduced by Senko, Marshall, and co-workers.",

author = "Kelleher, {Neil L.} and Senko, {Michael W.} and Siegel, {Marshall M.} and McLafferty, {Fred W.}",

note = "Funding Information: The authors thank Kiran Khandke fchondroitinase samples), Alan Marshall (National High-Field FT-ICR MS Facility, NHMFL, NSF CHE-94-130081, the National Institutes of Health (FWM, GM16609; NLK, Cell & Molecular Biology Training Grant No. 08-T2GM07273), and the American Chemical Society (NLK, Division of Analytical Chemistry full fellowship sponsored by Perkin-Elmer Corporation).",

year = "1997",

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doi = "10.1016/S1044-0305(97)00060-3",

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T1 - Unit resolution mass spectra of 112 kDa molecules with 3 Da accuracy

AU - Kelleher, Neil L.

AU - Senko, Michael W.

AU - Siegel, Marshall M.

AU - McLafferty, Fred W.

N1 - Funding Information: The authors thank Kiran Khandke fchondroitinase samples), Alan Marshall (National High-Field FT-ICR MS Facility, NHMFL, NSF CHE-94-130081, the National Institutes of Health (FWM, GM16609; NLK, Cell & Molecular Biology Training Grant No. 08-T2GM07273), and the American Chemical Society (NLK, Division of Analytical Chemistry full fellowship sponsored by Perkin-Elmer Corporation).

PY - 1997/4

Y1 - 1997/4

N2 - Previously, the highest molecular weight for a sample yielding a unit resolution mass spectrum was 67 kDa (marginal at 86 kDa), obtained with a 6.2 T Fourier-transform mass spectrometer with electrospray ionization. Now with a 9.4 T instrument, resolving power of 170,000 has been achieved for chondroitinase I (997 amino acids) and II (990 amino acids), making possible molecular weight assignments of 112,509 and 111,714, respectively, versus 112,508 and 111,713 calculated. Assisting these assignments was the noise reduction in the resolved isotopic peaks achieved by the time domain data sampling technique introduced by Senko, Marshall, and co-workers.

AB - Previously, the highest molecular weight for a sample yielding a unit resolution mass spectrum was 67 kDa (marginal at 86 kDa), obtained with a 6.2 T Fourier-transform mass spectrometer with electrospray ionization. Now with a 9.4 T instrument, resolving power of 170,000 has been achieved for chondroitinase I (997 amino acids) and II (990 amino acids), making possible molecular weight assignments of 112,509 and 111,714, respectively, versus 112,508 and 111,713 calculated. Assisting these assignments was the noise reduction in the resolved isotopic peaks achieved by the time domain data sampling technique introduced by Senko, Marshall, and co-workers.

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Unit resolution mass spectra of 112 kDa molecules with 3 Da accuracy

Abstract

Funding

ASJC Scopus subject areas

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