UNIVERSAL PHOSPHORAMIDITE FOR THE PREPARATION OF MODIFIED BIOMOLECULES AND SURFACES

This invention provides a novel reagent for use in oligonucleotide and related structures, and methods for modifying a wide range of biomolecules in order to attach them to diverse types of surfaces. Abstract The invention describes a novel non-nucleoside tosyl phosphoramidite for modification of biomolecules, like oligonucleotides, from both the 3' and 5' ends. The phosphoramidite is prepared conveniently from commercially available starting materials and can be applied to modify molecules post-synthesis or during the synthesis of oligonucleotides to prepare tosylated oligos bearing a single or multiples of tosyl functionalities. The tosyl-modified biomolecules can then be attached on modified surfaces like gold, glass, polymer, metal and plastic.