Up-regulation of vitamin B1 homeostasis genes in breast cancer

Jason A. Zastre*, Bradley S. Hanberry, Rebecca L. Sweet, A. Cary McGinnis, Kristen R. Venuti, Michael G. Bartlett, Rajgopal Govindarajan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


An increased carbon flux and exploitation of metabolic pathways for the rapid generation of biosynthetic precursors is a common phenotype observed in breast cancer. To support this metabolic phenotype, cancer cells adaptively regulate the expression of glycolytic enzymes and nutrient transporters. However, activity of several enzymes involved in glucose metabolism requires an adequate supply of cofactors. In particular, vitamin B1 (thiamine) is utilized as an essential cofactor for metabolic enzymes that intersect at critical junctions within the glycolytic network. Intracellular availability of thiamine is facilitated by the activity of thiamine transporters and thiamine pyrophosphokinase-1 (TPK-1). Therefore, the objective of this study was to establish if the cellular determinants regulating thiamine homeostasis differ between breast cancer and normal breast epithelia. Employing cDNA arrays of breast cancer and normal breast epithelial tissues, SLC19A2, SLC25A19 and TPK-1 were found to be significantly up-regulated. Similarly, up-regulation was also observed in breast cancer cell lines compared to human mammary epithelial cells. Thiamine transport assays and quantitation of intracellular thiamine and thiamine pyrophosphate established a significantly greater extent of thiamine transport and free thiamine levels in breast cancer cell lines compared to human mammary epithelial cells. Overall, these findings demonstrate an adaptive response by breast cancer cells to increase cellular availability of thiamine.

Original languageEnglish (US)
Pages (from-to)1616-1624
Number of pages9
JournalJournal of Nutritional Biochemistry
Issue number9
StatePublished - Sep 2013
Externally publishedYes


  • Breast cancer
  • Metabolism
  • Thiamine
  • Transporter
  • Vitamin

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology
  • Nutrition and Dietetics
  • Clinical Biochemistry


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