TY - JOUR
T1 - Upconversion Nanoprobes for the Ratiometric Luminescent Sensing of Nitric Oxide
AU - Wang, Ningning
AU - Yu, Xinyan
AU - Zhang, Ke
AU - Mirkin, Chad A.
AU - Li, Jishan
N1 - Funding Information:
This work was supported by NSFC (21475036, 21775035, 21527810) and the Hunan Provincial Natural Science Foundation (2016JJ1005).
Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/9/13
Y1 - 2017/9/13
N2 - By taking advantage of the optical properties of upconversion nanoparticles (UCNPs), we have designed a luminescence ratiometric nanosensor for measuring nitric oxide (NO) in biological fluids, live cells, and tissues. This nanoconjugate consists of a UCNP core with two strong fluorescence emission peaks at 540 and 656 nm as the upconversion fluorophore, NO-reactive rhodamine B-derived molecules (RdMs) encapsulated within the mesopores of the mSiO2 shell, and a β-cyclodextrin (βCD) layer on the exterior of the particle. Reaction of the analyte with the O-phenylenediamine of the RdM induces opening of the spiro-ring and is accompanied by an appearance of a strong rhodamine B (RdB) absorption band between 500 and 600 nm, which has spectral overlap with the green emission (540 nm) of the UCNPs. This results in an increase in the I656/I540 ratio and quantitatively correlates with [NO]. The assay is validated under clean buffer conditions as well as inserum and liver tissue slices obtained from mouse models.
AB - By taking advantage of the optical properties of upconversion nanoparticles (UCNPs), we have designed a luminescence ratiometric nanosensor for measuring nitric oxide (NO) in biological fluids, live cells, and tissues. This nanoconjugate consists of a UCNP core with two strong fluorescence emission peaks at 540 and 656 nm as the upconversion fluorophore, NO-reactive rhodamine B-derived molecules (RdMs) encapsulated within the mesopores of the mSiO2 shell, and a β-cyclodextrin (βCD) layer on the exterior of the particle. Reaction of the analyte with the O-phenylenediamine of the RdM induces opening of the spiro-ring and is accompanied by an appearance of a strong rhodamine B (RdB) absorption band between 500 and 600 nm, which has spectral overlap with the green emission (540 nm) of the UCNPs. This results in an increase in the I656/I540 ratio and quantitatively correlates with [NO]. The assay is validated under clean buffer conditions as well as inserum and liver tissue slices obtained from mouse models.
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U2 - 10.1021/jacs.7b06059
DO - 10.1021/jacs.7b06059
M3 - Article
C2 - 28844137
AN - SCOPUS:85029526975
SN - 0002-7863
VL - 139
SP - 12354
EP - 12357
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 36
ER -