Updated model of group A Streptococcus M proteins based on a comprehensive worldwide study

D. J. Mcmillan, P. A. Drèze, T. Vu, D. E. Bessen, J. Guglielmini, A. C. Steer, J. R. Carapetis, L. Van Melderen, K. S. Sriprakash, P. R. Smeesters*, Batzloff Michael Batzloff, Rebecca Towers, Herman Goossens, Surhbi Malhotra-Kumar, Luiza Guilherme, RosangelaTorres, Donald Low, Allison Mc Geer, Paula Krizova, Sawsan El Tayeb & 26 others Joe Kado, Mark van der Linden, Guliz rdem, Alon Moses, Ran Nir-Paz, Tadayoshi Ikebe, Haruo Watanabe, Samba Sow, Boubou Tamboura, Bard Kittang, José Melo-Cristino, Mario Ramirez, Monica Straut, Alexander Suvorov, Artem Totolian, Mark Engel, Bongani Mayosi, Andrew Whitelaw, Jessica Darenberg, Birgitta Henriques Normark, Chuan Chiang Ni, Jiunn Jong Wu, Aruni De Zoysa, Androulla Efstratiou, Stanford Shulman, Robert Tanz

*Corresponding author for this work

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Group A Streptococcus (GAS) M protein is an important virulence factor and potential vaccine antigen, and constitutes the basis for strain typing (emm-typing). Although >200 emm-types are characterized, structural data were obtained from only a limited number of emm-types. We aim to evaluate the sequence diversity of near-full-length M proteins from worldwide sources and analyse their structure, sequence conservation and classification. GAS isolates recovered from throughout the world during the last two decades underwent emm-typing and complete emm gene sequencing. Predicted amino acid sequence analyses, secondary structure predictions and vaccine epitope mapping were performed using MUSCLE and Geneious software. A total of 1086 isolates from 31 countries were analysed, representing 175 emm-types. emm-type is predictive of the whole protein structure, independent of geographical origin or clinical association. Findings of an emm-type paired with multiple, highly divergent central regions were not observed. M protein sequence length, the presence or absence of sequence repeats and predicted secondary structure were assessed in the context of the latest vaccine developments. Based on these global data, the M6 protein model is updated to a three representative M protein (M5, M80 and M77) model, to aid in epidemiological analysis, vaccine development and M protein-related pathogenesis studies.

Original languageEnglish (US)
JournalClinical Microbiology and Infection
Volume19
Issue number5
DOIs
StatePublished - Jan 1 2013

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Streptococcus
Vaccines
Proteins
Epitope Mapping
Protein Sequence Analysis
Virulence Factors
Software
Antigens
Genes

Keywords

  • Epidemiology
  • M protein
  • Streptococcus pyogenes
  • Typing
  • Vaccine
  • Virulence

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Mcmillan, D. J. ; Drèze, P. A. ; Vu, T. ; Bessen, D. E. ; Guglielmini, J. ; Steer, A. C. ; Carapetis, J. R. ; Van Melderen, L. ; Sriprakash, K. S. ; Smeesters, P. R. ; Michael Batzloff, Batzloff ; Towers, Rebecca ; Goossens, Herman ; Malhotra-Kumar, Surhbi ; Guilherme, Luiza ; RosangelaTorres ; Low, Donald ; Mc Geer, Allison ; Krizova, Paula ; El Tayeb, Sawsan ; Kado, Joe ; van der Linden, Mark ; rdem, Guliz ; Moses, Alon ; Nir-Paz, Ran ; Ikebe, Tadayoshi ; Watanabe, Haruo ; Sow, Samba ; Tamboura, Boubou ; Kittang, Bard ; Melo-Cristino, José ; Ramirez, Mario ; Straut, Monica ; Suvorov, Alexander ; Totolian, Artem ; Engel, Mark ; Mayosi, Bongani ; Whitelaw, Andrew ; Darenberg, Jessica ; Normark, Birgitta Henriques ; Chiang Ni, Chuan ; Wu, Jiunn Jong ; De Zoysa, Aruni ; Efstratiou, Androulla ; Shulman, Stanford ; Tanz, Robert. / Updated model of group A Streptococcus M proteins based on a comprehensive worldwide study. In: Clinical Microbiology and Infection. 2013 ; Vol. 19, No. 5.
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abstract = "Group A Streptococcus (GAS) M protein is an important virulence factor and potential vaccine antigen, and constitutes the basis for strain typing (emm-typing). Although >200 emm-types are characterized, structural data were obtained from only a limited number of emm-types. We aim to evaluate the sequence diversity of near-full-length M proteins from worldwide sources and analyse their structure, sequence conservation and classification. GAS isolates recovered from throughout the world during the last two decades underwent emm-typing and complete emm gene sequencing. Predicted amino acid sequence analyses, secondary structure predictions and vaccine epitope mapping were performed using MUSCLE and Geneious software. A total of 1086 isolates from 31 countries were analysed, representing 175 emm-types. emm-type is predictive of the whole protein structure, independent of geographical origin or clinical association. Findings of an emm-type paired with multiple, highly divergent central regions were not observed. M protein sequence length, the presence or absence of sequence repeats and predicted secondary structure were assessed in the context of the latest vaccine developments. Based on these global data, the M6 protein model is updated to a three representative M protein (M5, M80 and M77) model, to aid in epidemiological analysis, vaccine development and M protein-related pathogenesis studies.",
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author = "Mcmillan, {D. J.} and Dr{\`e}ze, {P. A.} and T. Vu and Bessen, {D. E.} and J. Guglielmini and Steer, {A. C.} and Carapetis, {J. R.} and {Van Melderen}, L. and Sriprakash, {K. S.} and Smeesters, {P. R.} and {Michael Batzloff}, Batzloff and Rebecca Towers and Herman Goossens and Surhbi Malhotra-Kumar and Luiza Guilherme and RosangelaTorres and Donald Low and {Mc Geer}, Allison and Paula Krizova and {El Tayeb}, Sawsan and Joe Kado and {van der Linden}, Mark and Guliz rdem and Alon Moses and Ran Nir-Paz and Tadayoshi Ikebe and Haruo Watanabe and Samba Sow and Boubou Tamboura and Bard Kittang and Jos{\'e} Melo-Cristino and Mario Ramirez and Monica Straut and Alexander Suvorov and Artem Totolian and Mark Engel and Bongani Mayosi and Andrew Whitelaw and Jessica Darenberg and Normark, {Birgitta Henriques} and {Chiang Ni}, Chuan and Wu, {Jiunn Jong} and {De Zoysa}, Aruni and Androulla Efstratiou and Stanford Shulman and Robert Tanz",
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Mcmillan, DJ, Drèze, PA, Vu, T, Bessen, DE, Guglielmini, J, Steer, AC, Carapetis, JR, Van Melderen, L, Sriprakash, KS, Smeesters, PR, Michael Batzloff, B, Towers, R, Goossens, H, Malhotra-Kumar, S, Guilherme, L, RosangelaTorres, Low, D, Mc Geer, A, Krizova, P, El Tayeb, S, Kado, J, van der Linden, M, rdem, G, Moses, A, Nir-Paz, R, Ikebe, T, Watanabe, H, Sow, S, Tamboura, B, Kittang, B, Melo-Cristino, J, Ramirez, M, Straut, M, Suvorov, A, Totolian, A, Engel, M, Mayosi, B, Whitelaw, A, Darenberg, J, Normark, BH, Chiang Ni, C, Wu, JJ, De Zoysa, A, Efstratiou, A, Shulman, S & Tanz, R 2013, 'Updated model of group A Streptococcus M proteins based on a comprehensive worldwide study', Clinical Microbiology and Infection, vol. 19, no. 5. https://doi.org/10.1111/1469-0691.12134

Updated model of group A Streptococcus M proteins based on a comprehensive worldwide study. / Mcmillan, D. J.; Drèze, P. A.; Vu, T.; Bessen, D. E.; Guglielmini, J.; Steer, A. C.; Carapetis, J. R.; Van Melderen, L.; Sriprakash, K. S.; Smeesters, P. R.; Michael Batzloff, Batzloff; Towers, Rebecca; Goossens, Herman; Malhotra-Kumar, Surhbi; Guilherme, Luiza; RosangelaTorres; Low, Donald; Mc Geer, Allison; Krizova, Paula; El Tayeb, Sawsan; Kado, Joe; van der Linden, Mark; rdem, Guliz; Moses, Alon; Nir-Paz, Ran; Ikebe, Tadayoshi; Watanabe, Haruo; Sow, Samba; Tamboura, Boubou; Kittang, Bard; Melo-Cristino, José; Ramirez, Mario; Straut, Monica; Suvorov, Alexander; Totolian, Artem; Engel, Mark; Mayosi, Bongani; Whitelaw, Andrew; Darenberg, Jessica; Normark, Birgitta Henriques; Chiang Ni, Chuan; Wu, Jiunn Jong; De Zoysa, Aruni; Efstratiou, Androulla; Shulman, Stanford; Tanz, Robert.

In: Clinical Microbiology and Infection, Vol. 19, No. 5, 01.01.2013.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Updated model of group A Streptococcus M proteins based on a comprehensive worldwide study

AU - Mcmillan, D. J.

AU - Drèze, P. A.

AU - Vu, T.

AU - Bessen, D. E.

AU - Guglielmini, J.

AU - Steer, A. C.

AU - Carapetis, J. R.

AU - Van Melderen, L.

AU - Sriprakash, K. S.

AU - Smeesters, P. R.

AU - Michael Batzloff, Batzloff

AU - Towers, Rebecca

AU - Goossens, Herman

AU - Malhotra-Kumar, Surhbi

AU - Guilherme, Luiza

AU - RosangelaTorres,

AU - Low, Donald

AU - Mc Geer, Allison

AU - Krizova, Paula

AU - El Tayeb, Sawsan

AU - Kado, Joe

AU - van der Linden, Mark

AU - rdem, Guliz

AU - Moses, Alon

AU - Nir-Paz, Ran

AU - Ikebe, Tadayoshi

AU - Watanabe, Haruo

AU - Sow, Samba

AU - Tamboura, Boubou

AU - Kittang, Bard

AU - Melo-Cristino, José

AU - Ramirez, Mario

AU - Straut, Monica

AU - Suvorov, Alexander

AU - Totolian, Artem

AU - Engel, Mark

AU - Mayosi, Bongani

AU - Whitelaw, Andrew

AU - Darenberg, Jessica

AU - Normark, Birgitta Henriques

AU - Chiang Ni, Chuan

AU - Wu, Jiunn Jong

AU - De Zoysa, Aruni

AU - Efstratiou, Androulla

AU - Shulman, Stanford

AU - Tanz, Robert

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Group A Streptococcus (GAS) M protein is an important virulence factor and potential vaccine antigen, and constitutes the basis for strain typing (emm-typing). Although >200 emm-types are characterized, structural data were obtained from only a limited number of emm-types. We aim to evaluate the sequence diversity of near-full-length M proteins from worldwide sources and analyse their structure, sequence conservation and classification. GAS isolates recovered from throughout the world during the last two decades underwent emm-typing and complete emm gene sequencing. Predicted amino acid sequence analyses, secondary structure predictions and vaccine epitope mapping were performed using MUSCLE and Geneious software. A total of 1086 isolates from 31 countries were analysed, representing 175 emm-types. emm-type is predictive of the whole protein structure, independent of geographical origin or clinical association. Findings of an emm-type paired with multiple, highly divergent central regions were not observed. M protein sequence length, the presence or absence of sequence repeats and predicted secondary structure were assessed in the context of the latest vaccine developments. Based on these global data, the M6 protein model is updated to a three representative M protein (M5, M80 and M77) model, to aid in epidemiological analysis, vaccine development and M protein-related pathogenesis studies.

AB - Group A Streptococcus (GAS) M protein is an important virulence factor and potential vaccine antigen, and constitutes the basis for strain typing (emm-typing). Although >200 emm-types are characterized, structural data were obtained from only a limited number of emm-types. We aim to evaluate the sequence diversity of near-full-length M proteins from worldwide sources and analyse their structure, sequence conservation and classification. GAS isolates recovered from throughout the world during the last two decades underwent emm-typing and complete emm gene sequencing. Predicted amino acid sequence analyses, secondary structure predictions and vaccine epitope mapping were performed using MUSCLE and Geneious software. A total of 1086 isolates from 31 countries were analysed, representing 175 emm-types. emm-type is predictive of the whole protein structure, independent of geographical origin or clinical association. Findings of an emm-type paired with multiple, highly divergent central regions were not observed. M protein sequence length, the presence or absence of sequence repeats and predicted secondary structure were assessed in the context of the latest vaccine developments. Based on these global data, the M6 protein model is updated to a three representative M protein (M5, M80 and M77) model, to aid in epidemiological analysis, vaccine development and M protein-related pathogenesis studies.

KW - Epidemiology

KW - M protein

KW - Streptococcus pyogenes

KW - Typing

KW - Vaccine

KW - Virulence

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JO - Clinical Microbiology and Infection

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