Purpose: The aim of this study is to search for new therapeutic targets for atypical teratoid-rhabdoid tumors (ATRT). Methods: To achieve this, we compared the expression of 365 microRNAs among ATRT, medulloblastomas, and normal brain. Results: MiR-221 and miR-222 were within the top differentially expressed microRNAs. The deregulated expression of miR221/222 was demonstrated to inhibit the expression of the tumor suppressor and inhibitor of cell cycle p27 Kip1. Here, we demonstrated the negative regulation of p27 Kip1 by miR-221/222 in ATRT using microarray, real-time reverse transcriptase polymerase chain reaction, and immunohistochemistry. Conclusion: As anti-miR therapy was recently proposed as an alternative treatment for cancer, these findings suggest that anti-miR-221/222 therapy might have therapeutic potential in ATRT.
- Atypical teratoid/rhabdoid tumors
ASJC Scopus subject areas
- Pediatrics, Perinatology, and Child Health
- Clinical Neurology