Uptake and metabolism of l-3,4-dihydroxyphenylalanine (dopa) in rat tissues

Lewis Landsberg; Howard L. Taubin

doi:10.1016/0006-2952(73)90146-9

Uptake and metabolism of l-3,4-dihydroxyphenylalanine (dopa) in rat tissues

Lewis Landsberg^*, Howard L. Taubin

^*Corresponding author for this work

Medicine, Endocrinology Division

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The uptake and metabolism of l-3,4-dihydroxyphenylalanine (dopa) in different rat tissues were studied after the administration of a single parenteral dose of l-dopa. The glandular portion of the rat stomach concentrated dopa in excess of plasma (6-fold), while cardiac dopa levels remained at or below the corresponding plasma level. The accumulation of dopa during the first 30 min after i.v. l-dopa was significantly greater in the glandular stomach than in the heart, duodenum, ileum or non-glandular stomach. The duodenum and ileum synthesized significantly more dopamine (DA) from dopa than did the other tissues. Studies with ³H-l-dopa revealed that the duodenum and ileum formed the most ³H-O-methylated metabolites. The total accumulation of tritium after ³H-l-dopa was greatest in the duodenum. Dopa and DA were localized to the cytosol in both glandular stomach and duodenum. Dopa and DA stores in the stomach were resistant to reserpine; 6-hydroxydopamine (6-OHDA) partially inhibited dopa and DA accumulation in glandular stomach but not in heart. It is concluded that the uptake and metabolism of dopa in different organs are heterogenous. The tissues of the gastrointestinal tract, in particular, are capable of taking up, metabolizing and storing dopa and dopa metabolites in high concentration. The uptake and metabolism of dopa are largely outside the adrenergic nerves.

Original language	English (US)
Pages (from-to)	2789-2800
Number of pages	12
Journal	Biochemical Pharmacology
Volume	22
Issue number	22
DOIs	https://doi.org/10.1016/0006-2952(73)90146-9
State	Published - Nov 15 1973

Funding

* A preliminary report was presented to the American Federation for Clinical Research (Eastern Section), January 12, 1973, Boston, Mass. (Clin. Res. 20, 872, 1972). t Supported in part by a grant (RR-76) from the General Clinical Research Center Program of the Division of Research Resources, National Institutes of Health, by a Harvard Medical School General Research Grant (NIH 5 501 RR5381-ll), by a Clinical Investigator and Research and Education Award of the Veterans Administration, and by the John A. Hartford Foundation. r Address reprint requests to: Lewis Landsberg, M.D., Harvard Medical Unit, Thorndike Memorial Laboratory, Boston City Hospital, 818 Harrison Ave., Boston, Mass., U.S.A. 2789

ASJC Scopus subject areas

Biochemistry
Pharmacology

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10.1016/0006-2952(73)90146-9

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title = "Uptake and metabolism of l-3,4-dihydroxyphenylalanine (dopa) in rat tissues",

abstract = "The uptake and metabolism of l-3,4-dihydroxyphenylalanine (dopa) in different rat tissues were studied after the administration of a single parenteral dose of l-dopa. The glandular portion of the rat stomach concentrated dopa in excess of plasma (6-fold), while cardiac dopa levels remained at or below the corresponding plasma level. The accumulation of dopa during the first 30 min after i.v. l-dopa was significantly greater in the glandular stomach than in the heart, duodenum, ileum or non-glandular stomach. The duodenum and ileum synthesized significantly more dopamine (DA) from dopa than did the other tissues. Studies with 3H-l-dopa revealed that the duodenum and ileum formed the most 3H-O-methylated metabolites. The total accumulation of tritium after 3H-l-dopa was greatest in the duodenum. Dopa and DA were localized to the cytosol in both glandular stomach and duodenum. Dopa and DA stores in the stomach were resistant to reserpine; 6-hydroxydopamine (6-OHDA) partially inhibited dopa and DA accumulation in glandular stomach but not in heart. It is concluded that the uptake and metabolism of dopa in different organs are heterogenous. The tissues of the gastrointestinal tract, in particular, are capable of taking up, metabolizing and storing dopa and dopa metabolites in high concentration. The uptake and metabolism of dopa are largely outside the adrenergic nerves.",

author = "Lewis Landsberg and Taubin, {Howard L.}",

note = "Funding Information: * A preliminary report was presented to the American Federation for Clinical Research (Eastern Section), January 12, 1973, Boston, Mass. (Clin. Res. 20, 872, 1972). t Supported in part by a grant (RR-76) from the General Clinical Research Center Program of the Division of Research Resources, National Institutes of Health, by a Harvard Medical School General Research Grant (NIH 5 501 RR5381-ll), by a Clinical Investigator and Research and Education Award of the Veterans Administration, and by the John A. Hartford Foundation. r Address reprint requests to: Lewis Landsberg, M.D., Harvard Medical Unit, Thorndike Memorial Laboratory, Boston City Hospital, 818 Harrison Ave., Boston, Mass., U.S.A. 2789",

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N1 - Funding Information: * A preliminary report was presented to the American Federation for Clinical Research (Eastern Section), January 12, 1973, Boston, Mass. (Clin. Res. 20, 872, 1972). t Supported in part by a grant (RR-76) from the General Clinical Research Center Program of the Division of Research Resources, National Institutes of Health, by a Harvard Medical School General Research Grant (NIH 5 501 RR5381-ll), by a Clinical Investigator and Research and Education Award of the Veterans Administration, and by the John A. Hartford Foundation. r Address reprint requests to: Lewis Landsberg, M.D., Harvard Medical Unit, Thorndike Memorial Laboratory, Boston City Hospital, 818 Harrison Ave., Boston, Mass., U.S.A. 2789

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N2 - The uptake and metabolism of l-3,4-dihydroxyphenylalanine (dopa) in different rat tissues were studied after the administration of a single parenteral dose of l-dopa. The glandular portion of the rat stomach concentrated dopa in excess of plasma (6-fold), while cardiac dopa levels remained at or below the corresponding plasma level. The accumulation of dopa during the first 30 min after i.v. l-dopa was significantly greater in the glandular stomach than in the heart, duodenum, ileum or non-glandular stomach. The duodenum and ileum synthesized significantly more dopamine (DA) from dopa than did the other tissues. Studies with 3H-l-dopa revealed that the duodenum and ileum formed the most 3H-O-methylated metabolites. The total accumulation of tritium after 3H-l-dopa was greatest in the duodenum. Dopa and DA were localized to the cytosol in both glandular stomach and duodenum. Dopa and DA stores in the stomach were resistant to reserpine; 6-hydroxydopamine (6-OHDA) partially inhibited dopa and DA accumulation in glandular stomach but not in heart. It is concluded that the uptake and metabolism of dopa in different organs are heterogenous. The tissues of the gastrointestinal tract, in particular, are capable of taking up, metabolizing and storing dopa and dopa metabolites in high concentration. The uptake and metabolism of dopa are largely outside the adrenergic nerves.

AB - The uptake and metabolism of l-3,4-dihydroxyphenylalanine (dopa) in different rat tissues were studied after the administration of a single parenteral dose of l-dopa. The glandular portion of the rat stomach concentrated dopa in excess of plasma (6-fold), while cardiac dopa levels remained at or below the corresponding plasma level. The accumulation of dopa during the first 30 min after i.v. l-dopa was significantly greater in the glandular stomach than in the heart, duodenum, ileum or non-glandular stomach. The duodenum and ileum synthesized significantly more dopamine (DA) from dopa than did the other tissues. Studies with 3H-l-dopa revealed that the duodenum and ileum formed the most 3H-O-methylated metabolites. The total accumulation of tritium after 3H-l-dopa was greatest in the duodenum. Dopa and DA were localized to the cytosol in both glandular stomach and duodenum. Dopa and DA stores in the stomach were resistant to reserpine; 6-hydroxydopamine (6-OHDA) partially inhibited dopa and DA accumulation in glandular stomach but not in heart. It is concluded that the uptake and metabolism of dopa in different organs are heterogenous. The tissues of the gastrointestinal tract, in particular, are capable of taking up, metabolizing and storing dopa and dopa metabolites in high concentration. The uptake and metabolism of dopa are largely outside the adrenergic nerves.

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Uptake and metabolism of l-3,4-dihydroxyphenylalanine (dopa) in rat tissues

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