Uptake-sequence-independent DNA transformation exists in Neisseria gonorrhoeae

Susan Boyle-Vavra; H. Steven Seifert

doi:10.1099/13500872-142-10-2839

Uptake-sequence-independent DNA transformation exists in Neisseria gonorrhoeae

Susan Boyle-Vavra, H. Steven Seifert^*

^*Corresponding author for this work

Microbiology-Immunology

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

A DNA transformation dose-response curve of piliated (P⁺) gonococci with the use of cloned DNA containing a pilE2-cat fusion showed saturation at high and low levels of transforming DNA. At low DNA concentrations, transformation of the P⁺ strain MS11-A was effectively inhibited by a 1000-fold molar excess of the gonococcal transformation uptake sequence (GCUS). The same molar excess of the GCUS did not inhibit transformation of MS11-A at high DNA concentrations. In MS11-B2, a nonpiliated (P^-), pilin-nonproducing, isogenic variant of MS11-A, the GCUS did not inhibit transformation at any level of transforming DNA. These data suggest that two mechanisms of transformation exist in P⁺ cells: one which utilizes the GCUS and one which does not. In MS11-B2 P^- cells, no evidence was found for the presence of the GCUS-dependent mechanism, suggesting that transformation in this background occurs solely by the GCUS-independent mechanism.

Original language	English (US)
Pages (from-to)	2839-2845
Number of pages	7
Journal	Microbiology
Volume	142
Issue number	10
DOIs	https://doi.org/10.1099/13500872-142-10-2839
State	Published - Oct 1996

Keywords

Competition
Dose-response
Pili

ASJC Scopus subject areas

Microbiology

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title = "Uptake-sequence-independent DNA transformation exists in Neisseria gonorrhoeae",

abstract = "A DNA transformation dose-response curve of piliated (P+) gonococci with the use of cloned DNA containing a pilE2-cat fusion showed saturation at high and low levels of transforming DNA. At low DNA concentrations, transformation of the P+ strain MS11-A was effectively inhibited by a 1000-fold molar excess of the gonococcal transformation uptake sequence (GCUS). The same molar excess of the GCUS did not inhibit transformation of MS11-A at high DNA concentrations. In MS11-B2, a nonpiliated (P-), pilin-nonproducing, isogenic variant of MS11-A, the GCUS did not inhibit transformation at any level of transforming DNA. These data suggest that two mechanisms of transformation exist in P+ cells: one which utilizes the GCUS and one which does not. In MS11-B2 P- cells, no evidence was found for the presence of the GCUS-dependent mechanism, suggesting that transformation in this background occurs solely by the GCUS-independent mechanism.",

keywords = "Competition, Dose-response, Pili",

author = "Susan Boyle-Vavra and Seifert, {H. Steven}",

year = "1996",

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T1 - Uptake-sequence-independent DNA transformation exists in Neisseria gonorrhoeae

AU - Boyle-Vavra, Susan

AU - Seifert, H. Steven

PY - 1996/10

Y1 - 1996/10

N2 - A DNA transformation dose-response curve of piliated (P+) gonococci with the use of cloned DNA containing a pilE2-cat fusion showed saturation at high and low levels of transforming DNA. At low DNA concentrations, transformation of the P+ strain MS11-A was effectively inhibited by a 1000-fold molar excess of the gonococcal transformation uptake sequence (GCUS). The same molar excess of the GCUS did not inhibit transformation of MS11-A at high DNA concentrations. In MS11-B2, a nonpiliated (P-), pilin-nonproducing, isogenic variant of MS11-A, the GCUS did not inhibit transformation at any level of transforming DNA. These data suggest that two mechanisms of transformation exist in P+ cells: one which utilizes the GCUS and one which does not. In MS11-B2 P- cells, no evidence was found for the presence of the GCUS-dependent mechanism, suggesting that transformation in this background occurs solely by the GCUS-independent mechanism.

AB - A DNA transformation dose-response curve of piliated (P+) gonococci with the use of cloned DNA containing a pilE2-cat fusion showed saturation at high and low levels of transforming DNA. At low DNA concentrations, transformation of the P+ strain MS11-A was effectively inhibited by a 1000-fold molar excess of the gonococcal transformation uptake sequence (GCUS). The same molar excess of the GCUS did not inhibit transformation of MS11-A at high DNA concentrations. In MS11-B2, a nonpiliated (P-), pilin-nonproducing, isogenic variant of MS11-A, the GCUS did not inhibit transformation at any level of transforming DNA. These data suggest that two mechanisms of transformation exist in P+ cells: one which utilizes the GCUS and one which does not. In MS11-B2 P- cells, no evidence was found for the presence of the GCUS-dependent mechanism, suggesting that transformation in this background occurs solely by the GCUS-independent mechanism.

KW - Competition

KW - Dose-response

KW - Pili

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Uptake-sequence-independent DNA transformation exists in Neisseria gonorrhoeae

Abstract

Keywords

ASJC Scopus subject areas

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