Urine levels of transforming growth factor-beta 1 in children with ureteropelvic junction obstruction

Lane S. Palmer*, Max Maizels, William E Kaplan, Casimir F. Firlit, Earl Y Cheng

*Corresponding author for this work

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Objectives. To determine if there are measurable quantities of transforming growth factor-beta 1 (TGF-β1) in the urine of children with either normal or pathologic conditions of the urinary tract, specifically vesicoureteral reflux (VUR) and ureteropelvic junction obstruction (UPJO). We also sought to determine if the urine TGF-β level could distinguish between renal obstruction and no obstruction. Methods. Preoperative bladder urine from consecutive patients undergoing pyeloplasty (UPJO group; n = 13), ureteral reimplantation (VUR group; n = 11), or circumcision/orchiopexy (control group; n = 19) as well as urine from the renal pelvis of the UPJO group was collected. The urine level of TGF-β1 was measured using a quantitative sandwich enzyme immunoassay technique. Results. Urine level of TGF-β1 was detected in each group: control (26.6 ± 6.3 pg/mL), reflux (22.1 ± 9.6), UPJO-pelvic urine (82.4 ± 19.3), UPJO-bladder urine (31.2 ± 8.2). The urine TGF-β1 concentration in pelvic urine in the UPJO group was significantly higher than that in bladder urine in children in the UPJO group (P = 0.03). TGF-β1 concentrations were similar from the bladder of children in all three study groups [P = NS). Conclusions. Urine TGF-β1 is detectable in children with normal and pathologic urinary tracts. The level of this urine marker is elevated in the renal pelvis of children with UPJO compared to the level in the bladder of either obstructed or nonobstructed upper urinary tracts.

Original languageEnglish (US)
Pages (from-to)769-773
Number of pages5
JournalUrology
Volume50
Issue number5
DOIs
StatePublished - Nov 1 1997

Fingerprint

Transforming Growth Factor beta
Urine
Urinary Bladder
Urinary Tract
Vesico-Ureteral Reflux
Kidney Pelvis
Orchiopexy
Control Groups
Replantation
Immunoenzyme Techniques
Kidney

ASJC Scopus subject areas

  • Urology

Cite this

Palmer, Lane S. ; Maizels, Max ; Kaplan, William E ; Firlit, Casimir F. ; Cheng, Earl Y. / Urine levels of transforming growth factor-beta 1 in children with ureteropelvic junction obstruction. In: Urology. 1997 ; Vol. 50, No. 5. pp. 769-773.
@article{d9f14d617343430582e46be336a72a31,
title = "Urine levels of transforming growth factor-beta 1 in children with ureteropelvic junction obstruction",
abstract = "Objectives. To determine if there are measurable quantities of transforming growth factor-beta 1 (TGF-β1) in the urine of children with either normal or pathologic conditions of the urinary tract, specifically vesicoureteral reflux (VUR) and ureteropelvic junction obstruction (UPJO). We also sought to determine if the urine TGF-β level could distinguish between renal obstruction and no obstruction. Methods. Preoperative bladder urine from consecutive patients undergoing pyeloplasty (UPJO group; n = 13), ureteral reimplantation (VUR group; n = 11), or circumcision/orchiopexy (control group; n = 19) as well as urine from the renal pelvis of the UPJO group was collected. The urine level of TGF-β1 was measured using a quantitative sandwich enzyme immunoassay technique. Results. Urine level of TGF-β1 was detected in each group: control (26.6 ± 6.3 pg/mL), reflux (22.1 ± 9.6), UPJO-pelvic urine (82.4 ± 19.3), UPJO-bladder urine (31.2 ± 8.2). The urine TGF-β1 concentration in pelvic urine in the UPJO group was significantly higher than that in bladder urine in children in the UPJO group (P = 0.03). TGF-β1 concentrations were similar from the bladder of children in all three study groups [P = NS). Conclusions. Urine TGF-β1 is detectable in children with normal and pathologic urinary tracts. The level of this urine marker is elevated in the renal pelvis of children with UPJO compared to the level in the bladder of either obstructed or nonobstructed upper urinary tracts.",
author = "Palmer, {Lane S.} and Max Maizels and Kaplan, {William E} and Firlit, {Casimir F.} and Cheng, {Earl Y}",
year = "1997",
month = "11",
day = "1",
doi = "10.1016/S0090-4295(97)00331-2",
language = "English (US)",
volume = "50",
pages = "769--773",
journal = "Urology",
issn = "0090-4295",
publisher = "Elsevier Inc.",
number = "5",

}

Urine levels of transforming growth factor-beta 1 in children with ureteropelvic junction obstruction. / Palmer, Lane S.; Maizels, Max; Kaplan, William E; Firlit, Casimir F.; Cheng, Earl Y.

In: Urology, Vol. 50, No. 5, 01.11.1997, p. 769-773.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Urine levels of transforming growth factor-beta 1 in children with ureteropelvic junction obstruction

AU - Palmer, Lane S.

AU - Maizels, Max

AU - Kaplan, William E

AU - Firlit, Casimir F.

AU - Cheng, Earl Y

PY - 1997/11/1

Y1 - 1997/11/1

N2 - Objectives. To determine if there are measurable quantities of transforming growth factor-beta 1 (TGF-β1) in the urine of children with either normal or pathologic conditions of the urinary tract, specifically vesicoureteral reflux (VUR) and ureteropelvic junction obstruction (UPJO). We also sought to determine if the urine TGF-β level could distinguish between renal obstruction and no obstruction. Methods. Preoperative bladder urine from consecutive patients undergoing pyeloplasty (UPJO group; n = 13), ureteral reimplantation (VUR group; n = 11), or circumcision/orchiopexy (control group; n = 19) as well as urine from the renal pelvis of the UPJO group was collected. The urine level of TGF-β1 was measured using a quantitative sandwich enzyme immunoassay technique. Results. Urine level of TGF-β1 was detected in each group: control (26.6 ± 6.3 pg/mL), reflux (22.1 ± 9.6), UPJO-pelvic urine (82.4 ± 19.3), UPJO-bladder urine (31.2 ± 8.2). The urine TGF-β1 concentration in pelvic urine in the UPJO group was significantly higher than that in bladder urine in children in the UPJO group (P = 0.03). TGF-β1 concentrations were similar from the bladder of children in all three study groups [P = NS). Conclusions. Urine TGF-β1 is detectable in children with normal and pathologic urinary tracts. The level of this urine marker is elevated in the renal pelvis of children with UPJO compared to the level in the bladder of either obstructed or nonobstructed upper urinary tracts.

AB - Objectives. To determine if there are measurable quantities of transforming growth factor-beta 1 (TGF-β1) in the urine of children with either normal or pathologic conditions of the urinary tract, specifically vesicoureteral reflux (VUR) and ureteropelvic junction obstruction (UPJO). We also sought to determine if the urine TGF-β level could distinguish between renal obstruction and no obstruction. Methods. Preoperative bladder urine from consecutive patients undergoing pyeloplasty (UPJO group; n = 13), ureteral reimplantation (VUR group; n = 11), or circumcision/orchiopexy (control group; n = 19) as well as urine from the renal pelvis of the UPJO group was collected. The urine level of TGF-β1 was measured using a quantitative sandwich enzyme immunoassay technique. Results. Urine level of TGF-β1 was detected in each group: control (26.6 ± 6.3 pg/mL), reflux (22.1 ± 9.6), UPJO-pelvic urine (82.4 ± 19.3), UPJO-bladder urine (31.2 ± 8.2). The urine TGF-β1 concentration in pelvic urine in the UPJO group was significantly higher than that in bladder urine in children in the UPJO group (P = 0.03). TGF-β1 concentrations were similar from the bladder of children in all three study groups [P = NS). Conclusions. Urine TGF-β1 is detectable in children with normal and pathologic urinary tracts. The level of this urine marker is elevated in the renal pelvis of children with UPJO compared to the level in the bladder of either obstructed or nonobstructed upper urinary tracts.

UR - http://www.scopus.com/inward/record.url?scp=0030722492&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030722492&partnerID=8YFLogxK

U2 - 10.1016/S0090-4295(97)00331-2

DO - 10.1016/S0090-4295(97)00331-2

M3 - Article

VL - 50

SP - 769

EP - 773

JO - Urology

JF - Urology

SN - 0090-4295

IS - 5

ER -