TY - JOUR
T1 - Urokinase is a positive regulator of epidermal proliferation in vivo
AU - Jensen, Pamela J.
AU - Lavker, Robert M.
N1 - Funding Information:
The generous gift of uPA–/–, tPA–/–, and wild-type strains of mice by Drs. Peter Carmeliet and Desire Collen (University of Leuven, Belgium) made this study possible. Grants from the National Institutes of Health to PJJ (RO1 AR42998) and RML (RO1 EY06769) supported this work. The authors acknowledge with gratitude the technical assistance of Ms. LeaAnn P. Lavy.
PY - 1999
Y1 - 1999
N2 - The epidermis is a self-renewing tissue that must maintain a basal proliferative rate as well as respond to various perturbing stimuli. Regulation of keratinocyte proliferation involves diverse molecules, including growth factors, ions, and hormones. We recently proposed that a proteinase, urokinase-type plasminogen activator (uPA) may be added to this list, based on correlative evidence linking expression of uPA and murine epidermal hyperproliferation. Here we report that, during the first 3 d of life, the epidermis from mice that bear a targeted deletion of the uPA gene has a significantly lower proliferative rate than the epidermis from wild- type mice. In contrast, proliferation in the matrix keratinocytes of the hair follicles is not decreased in neonatal UPA(-/-) mice. Vertical migration of keratinocytes during terminal differentiation was not affected. We therefore conclude that lack of uPA is associated with a decrease in epidermal proliferation.
AB - The epidermis is a self-renewing tissue that must maintain a basal proliferative rate as well as respond to various perturbing stimuli. Regulation of keratinocyte proliferation involves diverse molecules, including growth factors, ions, and hormones. We recently proposed that a proteinase, urokinase-type plasminogen activator (uPA) may be added to this list, based on correlative evidence linking expression of uPA and murine epidermal hyperproliferation. Here we report that, during the first 3 d of life, the epidermis from mice that bear a targeted deletion of the uPA gene has a significantly lower proliferative rate than the epidermis from wild- type mice. In contrast, proliferation in the matrix keratinocytes of the hair follicles is not decreased in neonatal UPA(-/-) mice. Vertical migration of keratinocytes during terminal differentiation was not affected. We therefore conclude that lack of uPA is associated with a decrease in epidermal proliferation.
UR - http://www.scopus.com/inward/record.url?scp=0032917127&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032917127&partnerID=8YFLogxK
U2 - 10.1046/j.1523-1747.1999.00494.x
DO - 10.1046/j.1523-1747.1999.00494.x
M3 - Article
C2 - 9989802
AN - SCOPUS:0032917127
VL - 112
SP - 240
EP - 244
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
SN - 0022-202X
IS - 2
ER -