Use of altered specificity mutants to probe a specific protein-protein interaction in differentiation: The GATA-1:FOG complex

John D. Crispino, Maya B. Lodish, Joel P. MacKay, Stuart H. Orkin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

203 Scopus citations

Abstract

GATA-1 and FOG (Friend of GATA-1) are each essential for erythroid and megakaryocyte development. FOG, a zinc finger protein, interacts with the amino (N) finger of GATA-1 and cooperates with GATA-1 to promote differentiation. To determine whether this interaction is critical for GATA- 1 action, we selected GATA-1 mutants in yeast that fail to interact with FOG but retain normal DNA binding, as well a compensatory FOG mutant that restores interaction. These novel GATA-1 mutants do not promote erythroid differentiation of GATA-1- erythroid cells. Differentiation is rescued by the second-site FOG mutant. Thus, interaction of FOG with GATA-1 is essential for the function of GATA-1 in erythroid differentiation. These findings provide a paradigm for dissecting protein-protein associations involved in mammalian development.

Original languageEnglish (US)
Pages (from-to)219-228
Number of pages10
JournalMolecular cell
Volume3
Issue number2
DOIs
StatePublished - Feb 1999

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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