Use of an enzyme-linked immunosorbent assay technique in the differential diagnosis of active pulmonary tuberculosis in humans

S. B. Kalish, R. C. Radin, John Phillip Phair, D. Levitz, C. R. Zeiss, E. Metzger

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Sera from patients with active pulmonary tuberculosis and pulmonary diseases frequently mimicking tuberculosis were assayed for immunoglobulin G antibody activity to purified protein derivative (PPD) by an enzyme-linked immunosorbent assay. A method of standardization was developed to limit assay variation. Patients with active pulmonary tuberculosis had a significantly greater mean level of antibody than had patients with atypical tuberculosis (P = 0.005), sarcoidosis (P = 0.0001), histoplasmosis (P = 0.004), blastomycosis (P = 0.008), or cryptococcosis (P = 0.017), patients who had received bacille Calmette-Guerin vaccination (P = 0.003) or who had a history of treated tuberculosis (P = 0.003), and PPD skin test-positive and skin test-negative control subjects (P = 0.001). This technique may have potential use as a rapid diagnostic aid in evaluating patients with suspected active pulmonary tuberculosis.

Original languageEnglish (US)
Pages (from-to)523-530
Number of pages8
JournalUnknown Journal
Volume147
Issue number3
DOIs
StatePublished - Jan 1 1983

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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