TY - JOUR
T1 - Use of molecular techniques for analysis of Ia structure-function relationships
AU - Germain, R. N.
AU - Bentley, D. M.
AU - Brown, M. A.
AU - Lechler, R.
AU - Margulies, D. H.
AU - Norcross, M. A.
AU - Tou, J.
PY - 1985
Y1 - 1985
N2 - An overview of a strategy for the molecular analysis of class II major histocompatibility complex (Ia) gene product structure-function relationships is presented, and results obtained to date by using this approach are summarized. The A(β), A(α), E(α), and E(β) genes have been cloned and sequenced to yield information on gene organization and primary protein sequence. Comparison of sequences from allelic forms of these genes show the NH2-terminal domain to be the locus of most intraspecies polymorphism. Transfection of I-A(α) and A(β) genes into B lymphoma cells or L cells has generated cells expressing the transfected gene products on their membrane. Such Ia+ transfectants present antigen to various T cells, which use the expressed I-A as a restriction element. Exon shuffling has shown the β1 domain of A(β) to play a predominant role in such restricted antigen recognition. Preliminary data refining this analysis to sites within β1, as well as data on control of α:β chain association, are reviewed, and future prospects for use of this approach in resolving questions of immunological interest are discussed.
AB - An overview of a strategy for the molecular analysis of class II major histocompatibility complex (Ia) gene product structure-function relationships is presented, and results obtained to date by using this approach are summarized. The A(β), A(α), E(α), and E(β) genes have been cloned and sequenced to yield information on gene organization and primary protein sequence. Comparison of sequences from allelic forms of these genes show the NH2-terminal domain to be the locus of most intraspecies polymorphism. Transfection of I-A(α) and A(β) genes into B lymphoma cells or L cells has generated cells expressing the transfected gene products on their membrane. Such Ia+ transfectants present antigen to various T cells, which use the expressed I-A as a restriction element. Exon shuffling has shown the β1 domain of A(β) to play a predominant role in such restricted antigen recognition. Preliminary data refining this analysis to sites within β1, as well as data on control of α:β chain association, are reviewed, and future prospects for use of this approach in resolving questions of immunological interest are discussed.
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M3 - Review article
C2 - 3926541
AN - SCOPUS:0022357372
SN - 0014-9446
VL - 44
SP - 2730
EP - 2735
JO - Federation proceedings
JF - Federation proceedings
IS - 11
ER -