Use of porcine acellular dermal matrix as a dermal substitute in rats

Anil Srivastava, Evangeline Z. DeSagun, Lawrence J. Jennings, Stephen Sethi, Anan Phuangsab, Marella Hanumadass, Hernan M. Reyes, Robert J. Walter*

*Corresponding author for this work

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Objective: To examine porcine acellular dermal matrix (ADM) as a xenogenic dermal substitute in a rat model. Summary Background Data: Acellular dermal matrix has been used in the treatment of full-thickness skin injuries as an allogenic dermal substitute providing a stable wound base in human and animal studies. Methods: Xenogenic and allogenic ADMs were produced by treating porcine or rat skin with Dispase and Triton X-100. Full-thickness skin defects (225 mm2) were created on the dorsum of rats (n = 29), porcine or rat ADMs were implanted in them, and these were overlain with ultrathin split-thickness skin grafts (STSGs). In two adjacent wounds, 0.005- or 0.017-inch-thick autografts were implanted. In other experiments, the antimicrobial agent used during ADM processing (azide or a mixture of antibiotics) and the orientation of the implanted ADM (papillary or reticular side of ADM facing the STSG) were studied. Grafts were evaluated grossly and histologically for 30 days after surgery. Results: Significant wound contraction was seen at 14, 20, and 30 days after surgery in wounds receiving xenogenic ADM, allogenic ADM, and thin STSGs. Contraction of wounds containing xenogenic ADM was significantly greater than that of wounds containing allogenic ADM at 30 days after surgery. Graft take was poor in wounds containing xenogenic ADM and moderately good in those containing allogenic ADM. Wound healing was not significantly affected by the antimicrobial agent used during ADM preparation or by the ADM orientation. Conclusion: Dispase-Triton-treated allogenic ADM was useful as a dermal substitute in full-thickness skin defects, but healing with xenogenic ADM was poor.

Original languageEnglish (US)
Pages (from-to)400-408
Number of pages9
JournalAnnals of surgery
Volume233
Issue number3
DOIs
StatePublished - Mar 14 2001

Fingerprint

Acellular Dermis
Artificial Skin
Swine
Wounds and Injuries
Skin
Transplants
Ambulatory Surgical Procedures
Anti-Infective Agents
Azides

ASJC Scopus subject areas

  • Surgery

Cite this

Srivastava, A., DeSagun, E. Z., Jennings, L. J., Sethi, S., Phuangsab, A., Hanumadass, M., ... Walter, R. J. (2001). Use of porcine acellular dermal matrix as a dermal substitute in rats. Annals of surgery, 233(3), 400-408. https://doi.org/10.1097/00000658-200103000-00015
Srivastava, Anil ; DeSagun, Evangeline Z. ; Jennings, Lawrence J. ; Sethi, Stephen ; Phuangsab, Anan ; Hanumadass, Marella ; Reyes, Hernan M. ; Walter, Robert J. / Use of porcine acellular dermal matrix as a dermal substitute in rats. In: Annals of surgery. 2001 ; Vol. 233, No. 3. pp. 400-408.
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abstract = "Objective: To examine porcine acellular dermal matrix (ADM) as a xenogenic dermal substitute in a rat model. Summary Background Data: Acellular dermal matrix has been used in the treatment of full-thickness skin injuries as an allogenic dermal substitute providing a stable wound base in human and animal studies. Methods: Xenogenic and allogenic ADMs were produced by treating porcine or rat skin with Dispase and Triton X-100. Full-thickness skin defects (225 mm2) were created on the dorsum of rats (n = 29), porcine or rat ADMs were implanted in them, and these were overlain with ultrathin split-thickness skin grafts (STSGs). In two adjacent wounds, 0.005- or 0.017-inch-thick autografts were implanted. In other experiments, the antimicrobial agent used during ADM processing (azide or a mixture of antibiotics) and the orientation of the implanted ADM (papillary or reticular side of ADM facing the STSG) were studied. Grafts were evaluated grossly and histologically for 30 days after surgery. Results: Significant wound contraction was seen at 14, 20, and 30 days after surgery in wounds receiving xenogenic ADM, allogenic ADM, and thin STSGs. Contraction of wounds containing xenogenic ADM was significantly greater than that of wounds containing allogenic ADM at 30 days after surgery. Graft take was poor in wounds containing xenogenic ADM and moderately good in those containing allogenic ADM. Wound healing was not significantly affected by the antimicrobial agent used during ADM preparation or by the ADM orientation. Conclusion: Dispase-Triton-treated allogenic ADM was useful as a dermal substitute in full-thickness skin defects, but healing with xenogenic ADM was poor.",
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Srivastava, A, DeSagun, EZ, Jennings, LJ, Sethi, S, Phuangsab, A, Hanumadass, M, Reyes, HM & Walter, RJ 2001, 'Use of porcine acellular dermal matrix as a dermal substitute in rats', Annals of surgery, vol. 233, no. 3, pp. 400-408. https://doi.org/10.1097/00000658-200103000-00015

Use of porcine acellular dermal matrix as a dermal substitute in rats. / Srivastava, Anil; DeSagun, Evangeline Z.; Jennings, Lawrence J.; Sethi, Stephen; Phuangsab, Anan; Hanumadass, Marella; Reyes, Hernan M.; Walter, Robert J.

In: Annals of surgery, Vol. 233, No. 3, 14.03.2001, p. 400-408.

Research output: Contribution to journalArticle

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T1 - Use of porcine acellular dermal matrix as a dermal substitute in rats

AU - Srivastava, Anil

AU - DeSagun, Evangeline Z.

AU - Jennings, Lawrence J.

AU - Sethi, Stephen

AU - Phuangsab, Anan

AU - Hanumadass, Marella

AU - Reyes, Hernan M.

AU - Walter, Robert J.

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N2 - Objective: To examine porcine acellular dermal matrix (ADM) as a xenogenic dermal substitute in a rat model. Summary Background Data: Acellular dermal matrix has been used in the treatment of full-thickness skin injuries as an allogenic dermal substitute providing a stable wound base in human and animal studies. Methods: Xenogenic and allogenic ADMs were produced by treating porcine or rat skin with Dispase and Triton X-100. Full-thickness skin defects (225 mm2) were created on the dorsum of rats (n = 29), porcine or rat ADMs were implanted in them, and these were overlain with ultrathin split-thickness skin grafts (STSGs). In two adjacent wounds, 0.005- or 0.017-inch-thick autografts were implanted. In other experiments, the antimicrobial agent used during ADM processing (azide or a mixture of antibiotics) and the orientation of the implanted ADM (papillary or reticular side of ADM facing the STSG) were studied. Grafts were evaluated grossly and histologically for 30 days after surgery. Results: Significant wound contraction was seen at 14, 20, and 30 days after surgery in wounds receiving xenogenic ADM, allogenic ADM, and thin STSGs. Contraction of wounds containing xenogenic ADM was significantly greater than that of wounds containing allogenic ADM at 30 days after surgery. Graft take was poor in wounds containing xenogenic ADM and moderately good in those containing allogenic ADM. Wound healing was not significantly affected by the antimicrobial agent used during ADM preparation or by the ADM orientation. Conclusion: Dispase-Triton-treated allogenic ADM was useful as a dermal substitute in full-thickness skin defects, but healing with xenogenic ADM was poor.

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Srivastava A, DeSagun EZ, Jennings LJ, Sethi S, Phuangsab A, Hanumadass M et al. Use of porcine acellular dermal matrix as a dermal substitute in rats. Annals of surgery. 2001 Mar 14;233(3):400-408. https://doi.org/10.1097/00000658-200103000-00015