Use of viral systems to study miRNA-Mediated regulation of gene expression in human cells

Forte Eleonora, Luftig Micah

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

MicroRNAs (miRNAs) are a class of small ∼22 nt regulatory RNAs that modulate mRNA expression in all multicellular eukaryotic organisms. Interestingly, viruses also encode miRNAs and these viral miRNAs target cellular and viral mRNAs to regulate virus replication and latent infection. In particular, herpesviruses encode a large number of miRNAs. Herpesvirus infection also changes the normal expression pro fi le of cellular miRNAs. New genetic tools have recently been generated to study the function of viral and cellular miRNAs in virus-infected cells. The creation of these reagents and use in Epstein-Barr virus-infected lymphoblastoid cell lines are discussed as a model viral system for the investigation of miRNA function.

Original languageEnglish (US)
Title of host publicationMicroRNA Protocols
EditorsShao-Yao Ying
Pages143-156
Number of pages14
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume936
ISSN (Print)1064-3745

Keywords

  • Epstein-Barr Virus
  • Mirna
  • Mrna Target
  • Sponge
  • Virus

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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