Using a microcomputer to convert percent response values to probits

Tom C. Krejcie*

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Certain relationships between dose/concentration and percent response/binding in various biochemical systems are sigmoidal rather than linear. This sigmoid relationship is considered linear only over the 20-80 percent range. Appropriate analysis of such data over nearly the entire response/binding range requires the conversion of the percent response data to probits or probability units, producing a linear relationship. While tables of probits are available, the author has incorporated the probability function for probit conversion into a commercially available spreadsheet program. Also included is the Rohlf and Sokal approximation for the area under the normal distribution curve (conversion of probits to cumulative percent). These functions allow use of a spreadsheet to automate the creation of dose-response curves, or standard curves as part of chemical assays (i.e., radioimmunoassay).

Original languageEnglish (US)
Pages (from-to)19-23
Number of pages5
JournalInternational Journal of Clinical Monitoring and Computing
Volume8
Issue number1
DOIs
StatePublished - Mar 1 1991

Keywords

  • RIA
  • dose-response
  • probit
  • spreadsheet

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Critical Care and Intensive Care Medicine

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