Using data-independent mass spectrometry to extend detectable dynamic range without prior fractionation

Young Ah Goo, David R. Goodlett

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Data-dependent acquisition (DDA) is widely used in shotgun proteomics to profile the protein components of complex samples. Although very popular, it presents major drawbacks in terms of detectable dynamic range. We developed a data-independent acquisition (DIA) method, called precursor acquisition independent from ion count (PAcIFIC), which shows increased protein identification as well as extended detectable dynamic range over DDA methods. In this chapter, we discuss recent advances that have made PAcIFIC faster, more accurate, and quantitative. Currently, the time needed to perform a PAcIFIC experiment can be reduced to less than 2 days using an ion trap mass spectrometer with an overall tandem mass spectral acquisition rate of 0.2 s or less.

Original languageEnglish (US)
Title of host publicationProteomic and Metabolomic Approaches to Biomarker Discovery
PublisherElsevier
Pages327-337
Number of pages11
ISBN (Electronic)9780128186077
DOIs
StatePublished - Jan 1 2019

Keywords

  • Data-independent acquisition (DIA)
  • Dynamic range
  • Precursor acquisition independent from ion count (PAcIFIC)
  • Pseudo-MRM
  • Quantification

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Goo, Y. A., & Goodlett, D. R. (2019). Using data-independent mass spectrometry to extend detectable dynamic range without prior fractionation. In Proteomic and Metabolomic Approaches to Biomarker Discovery (pp. 327-337). Elsevier. https://doi.org/10.1016/B978-0-12-818607-7.00018-9