Using electron microscopy to calculate optical properties of biological samples

Wenli Wu, Andrew J. Radosevich, Adam Eshein, The Quyen Nguyen, Ji Yi, Lusik Cherkezyan, Hemant K. Roy, Igal Szleifer, Vadim Backman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


The microscopic structural origins of optical properties in biological media are still not fully understood. Better understanding these origins can serve to improve the utility of existing techniques and facilitate the discovery of other novel techniques. We propose a novel analysis technique using electron microscopy (EM) to calculate optical properties of specific biological structures. This method is demonstrated with images of human epithelial colon cell nuclei. The spectrum of anisotropy factor g, the phase function and the shape factor D of the nuclei are calculated. The results show strong agreement with an independent study. This method provides a new way to extract the true phase function of biological samples and provides an independent validation for optical property measurement techniques.

Original languageEnglish (US)
Article number#275078
Pages (from-to)4749-4762
Number of pages14
JournalBiomedical Optics Express
Issue number11
StatePublished - Nov 1 2016


  • Biology and medicine
  • Light propagation in tissues
  • Light propagation in tissues
  • Optical diagnostics for medicine
  • Optical properties

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics


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