Using genetic and morphological analysis to distinguish endangered taxa from their hybrids with the cultivated exotic pest plant Lantana strigocamara (syn: Lantana camara)

Joyce Maschinski; Eileen Sirkin; Jeremie Fant

doi:10.1007/s10592-009-0035-6

Using genetic and morphological analysis to distinguish endangered taxa from their hybrids with the cultivated exotic pest plant Lantana strigocamara (syn: Lantana camara)

Joyce Maschinski^*, Eileen Sirkin, Jeremie Fant

^*Corresponding author for this work

Program in Biological Sciences-Chicago Botanic Garden

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Because highly invasive species can rapidly assimilate rare taxa, we questioned whether two Florida endangered Lantana depressa varieties existed 21 years after Sanders documented their widespread hybridization with exotic Lantana strigocamara, and whether morphological traits could accurately discriminate genetic individuals. Stepwise discriminant analysis of morphological characters discriminated the three taxa, correctly classifying 98, 91, 89% of L. strigocamara, L. depressa var. depressa, and var. floridana. Hybrids blurred taxonomic distinctions of varieties and reduced classification accuracy by 7-17%. Species-specific Random Fragment Length Polymorphism (RFLP-PCR) confirmed hybridization has occurred. Intersimple Sequence Repeat (ISSR) fingerprints analyzed with STRUCTURE identified three groups indicating introgression. Morphological traits significantly, but weakly correlated with q ratios (P = 0.0001; r² = 0.45). Although L. strigocamara introgression is widespread and ongoing, wild populations contain individuals that are predominantly L. depressa genome, supporting actions to remove adventive L. strigocamara, prevent its sale, and promote sales of genetically confirmed natives.

Original language	English (US)
Pages (from-to)	1607-1621
Number of pages	15
Journal	Conservation Genetics
Volume	11
Issue number	5
DOIs	https://doi.org/10.1007/s10592-009-0035-6
State	Published - 2010

Funding

Acknowledgments We dedicate this paper to the memory of Kathy Burks, an extraordinary native plant conservation advocate in Florida, who suggested we conduct this research. This work was funded by Florida Department of Agriculture and Consumer Services Division of Plant Industry Contracts #007997 and #009064. For assistance in the field and laboratory, we sincerely thank G. Gann, J. Possley, C. & D. Walters, C. Ettore, M. Petersen, B. Horwith, and A. Cantillo. We thank Javier Francisco-Ortega, the editor, and one anonymous reviewer for helpful comments on the manuscript.

Keywords

Endangered species
Hybridization
ISSR
Invasive species
STRUCTURE

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/s10592-009-0035-6

Cite this

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title = "Using genetic and morphological analysis to distinguish endangered taxa from their hybrids with the cultivated exotic pest plant Lantana strigocamara (syn: Lantana camara)",

abstract = "Because highly invasive species can rapidly assimilate rare taxa, we questioned whether two Florida endangered Lantana depressa varieties existed 21 years after Sanders documented their widespread hybridization with exotic Lantana strigocamara, and whether morphological traits could accurately discriminate genetic individuals. Stepwise discriminant analysis of morphological characters discriminated the three taxa, correctly classifying 98, 91, 89% of L. strigocamara, L. depressa var. depressa, and var. floridana. Hybrids blurred taxonomic distinctions of varieties and reduced classification accuracy by 7-17%. Species-specific Random Fragment Length Polymorphism (RFLP-PCR) confirmed hybridization has occurred. Intersimple Sequence Repeat (ISSR) fingerprints analyzed with STRUCTURE identified three groups indicating introgression. Morphological traits significantly, but weakly correlated with q ratios (P = 0.0001; r2 = 0.45). Although L. strigocamara introgression is widespread and ongoing, wild populations contain individuals that are predominantly L. depressa genome, supporting actions to remove adventive L. strigocamara, prevent its sale, and promote sales of genetically confirmed natives.",

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author = "Joyce Maschinski and Eileen Sirkin and Jeremie Fant",

note = "Funding Information: Acknowledgments We dedicate this paper to the memory of Kathy Burks, an extraordinary native plant conservation advocate in Florida, who suggested we conduct this research. This work was funded by Florida Department of Agriculture and Consumer Services Division of Plant Industry Contracts #007997 and #009064. For assistance in the field and laboratory, we sincerely thank G. Gann, J. Possley, C. & D. Walters, C. Ettore, M. Petersen, B. Horwith, and A. Cantillo. We thank Javier Francisco-Ortega, the editor, and one anonymous reviewer for helpful comments on the manuscript.",

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N1 - Funding Information: Acknowledgments We dedicate this paper to the memory of Kathy Burks, an extraordinary native plant conservation advocate in Florida, who suggested we conduct this research. This work was funded by Florida Department of Agriculture and Consumer Services Division of Plant Industry Contracts #007997 and #009064. For assistance in the field and laboratory, we sincerely thank G. Gann, J. Possley, C. & D. Walters, C. Ettore, M. Petersen, B. Horwith, and A. Cantillo. We thank Javier Francisco-Ortega, the editor, and one anonymous reviewer for helpful comments on the manuscript.

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N2 - Because highly invasive species can rapidly assimilate rare taxa, we questioned whether two Florida endangered Lantana depressa varieties existed 21 years after Sanders documented their widespread hybridization with exotic Lantana strigocamara, and whether morphological traits could accurately discriminate genetic individuals. Stepwise discriminant analysis of morphological characters discriminated the three taxa, correctly classifying 98, 91, 89% of L. strigocamara, L. depressa var. depressa, and var. floridana. Hybrids blurred taxonomic distinctions of varieties and reduced classification accuracy by 7-17%. Species-specific Random Fragment Length Polymorphism (RFLP-PCR) confirmed hybridization has occurred. Intersimple Sequence Repeat (ISSR) fingerprints analyzed with STRUCTURE identified three groups indicating introgression. Morphological traits significantly, but weakly correlated with q ratios (P = 0.0001; r2 = 0.45). Although L. strigocamara introgression is widespread and ongoing, wild populations contain individuals that are predominantly L. depressa genome, supporting actions to remove adventive L. strigocamara, prevent its sale, and promote sales of genetically confirmed natives.

AB - Because highly invasive species can rapidly assimilate rare taxa, we questioned whether two Florida endangered Lantana depressa varieties existed 21 years after Sanders documented their widespread hybridization with exotic Lantana strigocamara, and whether morphological traits could accurately discriminate genetic individuals. Stepwise discriminant analysis of morphological characters discriminated the three taxa, correctly classifying 98, 91, 89% of L. strigocamara, L. depressa var. depressa, and var. floridana. Hybrids blurred taxonomic distinctions of varieties and reduced classification accuracy by 7-17%. Species-specific Random Fragment Length Polymorphism (RFLP-PCR) confirmed hybridization has occurred. Intersimple Sequence Repeat (ISSR) fingerprints analyzed with STRUCTURE identified three groups indicating introgression. Morphological traits significantly, but weakly correlated with q ratios (P = 0.0001; r2 = 0.45). Although L. strigocamara introgression is widespread and ongoing, wild populations contain individuals that are predominantly L. depressa genome, supporting actions to remove adventive L. strigocamara, prevent its sale, and promote sales of genetically confirmed natives.

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Using genetic and morphological analysis to distinguish endangered taxa from their hybrids with the cultivated exotic pest plant Lantana strigocamara (syn: Lantana camara)

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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