TY - JOUR
T1 - Using genetic and morphological analysis to distinguish endangered taxa from their hybrids with the cultivated exotic pest plant Lantana strigocamara (syn
T2 - Lantana camara)
AU - Maschinski, Joyce
AU - Sirkin, Eileen
AU - Fant, Jeremie
N1 - Funding Information:
Acknowledgments We dedicate this paper to the memory of Kathy Burks, an extraordinary native plant conservation advocate in Florida, who suggested we conduct this research. This work was funded by Florida Department of Agriculture and Consumer Services Division of Plant Industry Contracts #007997 and #009064. For assistance in the field and laboratory, we sincerely thank G. Gann, J. Possley, C. & D. Walters, C. Ettore, M. Petersen, B. Horwith, and A. Cantillo. We thank Javier Francisco-Ortega, the editor, and one anonymous reviewer for helpful comments on the manuscript.
PY - 2010
Y1 - 2010
N2 - Because highly invasive species can rapidly assimilate rare taxa, we questioned whether two Florida endangered Lantana depressa varieties existed 21 years after Sanders documented their widespread hybridization with exotic Lantana strigocamara, and whether morphological traits could accurately discriminate genetic individuals. Stepwise discriminant analysis of morphological characters discriminated the three taxa, correctly classifying 98, 91, 89% of L. strigocamara, L. depressa var. depressa, and var. floridana. Hybrids blurred taxonomic distinctions of varieties and reduced classification accuracy by 7-17%. Species-specific Random Fragment Length Polymorphism (RFLP-PCR) confirmed hybridization has occurred. Intersimple Sequence Repeat (ISSR) fingerprints analyzed with STRUCTURE identified three groups indicating introgression. Morphological traits significantly, but weakly correlated with q ratios (P = 0.0001; r2 = 0.45). Although L. strigocamara introgression is widespread and ongoing, wild populations contain individuals that are predominantly L. depressa genome, supporting actions to remove adventive L. strigocamara, prevent its sale, and promote sales of genetically confirmed natives.
AB - Because highly invasive species can rapidly assimilate rare taxa, we questioned whether two Florida endangered Lantana depressa varieties existed 21 years after Sanders documented their widespread hybridization with exotic Lantana strigocamara, and whether morphological traits could accurately discriminate genetic individuals. Stepwise discriminant analysis of morphological characters discriminated the three taxa, correctly classifying 98, 91, 89% of L. strigocamara, L. depressa var. depressa, and var. floridana. Hybrids blurred taxonomic distinctions of varieties and reduced classification accuracy by 7-17%. Species-specific Random Fragment Length Polymorphism (RFLP-PCR) confirmed hybridization has occurred. Intersimple Sequence Repeat (ISSR) fingerprints analyzed with STRUCTURE identified three groups indicating introgression. Morphological traits significantly, but weakly correlated with q ratios (P = 0.0001; r2 = 0.45). Although L. strigocamara introgression is widespread and ongoing, wild populations contain individuals that are predominantly L. depressa genome, supporting actions to remove adventive L. strigocamara, prevent its sale, and promote sales of genetically confirmed natives.
KW - Endangered species
KW - Hybridization
KW - ISSR
KW - Invasive species
KW - STRUCTURE
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U2 - 10.1007/s10592-009-0035-6
DO - 10.1007/s10592-009-0035-6
M3 - Article
AN - SCOPUS:77956886812
SN - 1566-0621
VL - 11
SP - 1607
EP - 1621
JO - Conservation Genetics
JF - Conservation Genetics
IS - 5
ER -