Using isotope-labeled proteins and sum frequency generation vibrational spectroscopy to study protein adsorption

Jie Wang, Matthew L. Clarke, Yongbo Zhang, Xiaoyun Chen, Zhan Chen*

*Corresponding author for this work

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

The application of sum frequency generation (SFG) vibrational spectroscopy to investigate protein behavior through the study of deuterated proteins has been demonstrated for the first time, showing the overall importance and promise of this combination of techniques. Using deuterated proteins and SFG, we report the feasibility of molecular level studies on the responses of polymer surfaces to protein adsorption, the dynamic process of protein adsorption, and protein mixture adsorption. We also demonstrate that the combination of selective protein isotope labeling and the SFG technique can be expanded in the future to elucidate more detailed structures of adsorbed proteins.

Original languageEnglish (US)
Pages (from-to)7862-7866
Number of pages5
JournalLangmuir
Volume19
Issue number19
DOIs
StatePublished - Sep 16 2003

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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