Using mass spectrometry to characterize self-assembled monolayers presenting peptides, proteins, and carbohydrates

Jing Su; Milan Mrksich

doi:10.1002/anie.200290026

Using mass spectrometry to characterize self-assembled monolayers presenting peptides, proteins, and carbohydrates

Jing Su, Milan Mrksich^*

^*Corresponding author for this work

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

149 Scopus citations

Abstract

Peptide and carbohydrate biochips can be assayed with MALDI mass spectrometry. Self-assembled monolayers (SAMs) that are modified with ligands provide a platform for identifying selective protein-ligand and enzyme-substrate interactions. MALDI provides a rapid and sensitive method for detection and avoids the need to label protein analytes with fluorescent or radioactive tags. The picture shows the mass spectrum of a SAM presenting a carbohydrate.

Original language	English (US)
Pages (from-to)	4715-4718
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	41
Issue number	24
DOIs	https://doi.org/10.1002/anie.200290026
State	Published - Dec 16 2002

Keywords

Biochips
Carbohydrates
Glycosylation
Mass spectrometry
Monolayers

ASJC Scopus subject areas

Catalysis
Chemistry(all)

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10.1002/anie.200290026

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KW - Glycosylation

KW - Mass spectrometry

KW - Monolayers

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Using mass spectrometry to characterize self-assembled monolayers presenting peptides, proteins, and carbohydrates

Abstract

Keywords

ASJC Scopus subject areas

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