Using Microfluidics and Imaging SAMDI-MS to Characterize Reaction Kinetics

Jennifer Grant; Patrick T. O'Kane; Blaise R. Kimmel; Milan Mrksich

doi:10.1021/acscentsci.8b00867

Using Microfluidics and Imaging SAMDI-MS to Characterize Reaction Kinetics

Jennifer Grant, Patrick T. O'Kane, Blaise R. Kimmel, Milan Mrksich^*

^*Corresponding author for this work

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Microfluidic platforms have enabled the simplification of biochemical assays with a significant reduction in the use of reagents, yet the current methods available for analyzing reaction products can limit applications of these approaches. This paper demonstrates a simple microfluidic device that incorporates a functionalized self-assembled monolayer to measure the rate constant for a chemical reaction. The device mixes the reactants and allows them to selectively immobilize to the monolayer at the base of a microfluidic channel in a time-dependent manner as they flow down the channel. Imaging self-assembled monolayers for matrix-assisted laser desorption/ionization mass spectrometry (iSAMDI-MS) is used to acquire a quantitative image representing the time-resolved progress of the reaction as it flowed through the channel. Knowledge of the surface immobilization chemistry and the fluid front characteristics allows for the determination of the chemical reaction rate constant. This approach widens the applicability of microfluidics for chemical reaction monitoring and establishes a label-free method for studying processes that occur within a dispersive regime.

Original language	English (US)
Pages (from-to)	486-493
Number of pages	8
Journal	ACS Central Science
Volume	5
Issue number	3
DOIs	https://doi.org/10.1021/acscentsci.8b00867
State	Published - Mar 27 2019

Funding

J.G. and B.R.K. were supported by the National Science Foundation Graduate Research Fellowship under Grants DGE-1324585 and DGE-1842165, respectively. We acknowledge support from the Department of the Defense, Defense Threat Reduction Agency HDTRA1-15-1-0052. J.G. and B.R.K. were supported by the National Science Foundation Graduate Research Fellowship under Grants DGE-1324585 and DGE-1842165, respectively. We acknowledge support from the Department of the Defense, Defense Threat Reduction Agency HDTRA1-15-1-0052. This work used facilities of the Integrated Molecular Structure Education and Research Center, the 3D Printing & Rapid Prototyping Lab, the Structural Biology Core, and the Research Shop Instrumentation Design, Engineering & Production.

ASJC Scopus subject areas

General Chemistry
General Chemical Engineering

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10.1021/acscentsci.8b00867

Cite this

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abstract = "Microfluidic platforms have enabled the simplification of biochemical assays with a significant reduction in the use of reagents, yet the current methods available for analyzing reaction products can limit applications of these approaches. This paper demonstrates a simple microfluidic device that incorporates a functionalized self-assembled monolayer to measure the rate constant for a chemical reaction. The device mixes the reactants and allows them to selectively immobilize to the monolayer at the base of a microfluidic channel in a time-dependent manner as they flow down the channel. Imaging self-assembled monolayers for matrix-assisted laser desorption/ionization mass spectrometry (iSAMDI-MS) is used to acquire a quantitative image representing the time-resolved progress of the reaction as it flowed through the channel. Knowledge of the surface immobilization chemistry and the fluid front characteristics allows for the determination of the chemical reaction rate constant. This approach widens the applicability of microfluidics for chemical reaction monitoring and establishes a label-free method for studying processes that occur within a dispersive regime.",

author = "Jennifer Grant and O'Kane, {Patrick T.} and Kimmel, {Blaise R.} and Milan Mrksich",

note = "Funding Information: J.G. and B.R.K. were supported by the National Science Foundation Graduate Research Fellowship under Grants DGE-1324585 and DGE-1842165, respectively. We acknowledge support from the Department of the Defense, Defense Threat Reduction Agency HDTRA1-15-1-0052. Funding Information: J.G. and B.R.K. were supported by the National Science Foundation Graduate Research Fellowship under Grants DGE-1324585 and DGE-1842165, respectively. We acknowledge support from the Department of the Defense, Defense Threat Reduction Agency HDTRA1-15-1-0052. This work used facilities of the Integrated Molecular Structure Education and Research Center, the 3D Printing & Rapid Prototyping Lab, the Structural Biology Core, and the Research Shop Instrumentation Design, Engineering & Production. Publisher Copyright: {\textcopyright} 2019 American Chemical Society.",

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N2 - Microfluidic platforms have enabled the simplification of biochemical assays with a significant reduction in the use of reagents, yet the current methods available for analyzing reaction products can limit applications of these approaches. This paper demonstrates a simple microfluidic device that incorporates a functionalized self-assembled monolayer to measure the rate constant for a chemical reaction. The device mixes the reactants and allows them to selectively immobilize to the monolayer at the base of a microfluidic channel in a time-dependent manner as they flow down the channel. Imaging self-assembled monolayers for matrix-assisted laser desorption/ionization mass spectrometry (iSAMDI-MS) is used to acquire a quantitative image representing the time-resolved progress of the reaction as it flowed through the channel. Knowledge of the surface immobilization chemistry and the fluid front characteristics allows for the determination of the chemical reaction rate constant. This approach widens the applicability of microfluidics for chemical reaction monitoring and establishes a label-free method for studying processes that occur within a dispersive regime.

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Using Microfluidics and Imaging SAMDI-MS to Characterize Reaction Kinetics

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