Using the inherent chemistry of the endothelin-1 peptide to develop a rapid assay for pre-transplant donor lung assessment

Andrew T. Sage; Xiaohui Bai; Marcelo Cypel; Mingyao Liu; Shaf Keshavjee; Shana O. Kelley

doi:10.1039/c5an01536g

Using the inherent chemistry of the endothelin-1 peptide to develop a rapid assay for pre-transplant donor lung assessment

Andrew T. Sage, Xiaohui Bai, Marcelo Cypel, Mingyao Liu, Shaf Keshavjee, Shana O. Kelley^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Endothelin-1 is a potent vasoconstrictive peptide that plays an important role in ex vivo lung perfusion. ET-1 expression levels are predictive of lung transplant outcomes and represent a valuable monitoring tool for surgeons; however, traditional techniques that measure [ET-1] are not suitable for the transplant setting. Herein, we demonstrate a new assay that rapidly measures ET-1 peptide levels in lung perfusate.

Original language	English (US)
Pages (from-to)	8092-8096
Number of pages	5
Journal	Analyst
Volume	140
Issue number	24
DOIs	https://doi.org/10.1039/c5an01536g
State	Published - Dec 21 2015
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry

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abstract = "Endothelin-1 is a potent vasoconstrictive peptide that plays an important role in ex vivo lung perfusion. ET-1 expression levels are predictive of lung transplant outcomes and represent a valuable monitoring tool for surgeons; however, traditional techniques that measure [ET-1] are not suitable for the transplant setting. Herein, we demonstrate a new assay that rapidly measures ET-1 peptide levels in lung perfusate.",

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AU - Liu, Mingyao

AU - Keshavjee, Shaf

AU - Kelley, Shana O.

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PY - 2015/12/21

Y1 - 2015/12/21

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AB - Endothelin-1 is a potent vasoconstrictive peptide that plays an important role in ex vivo lung perfusion. ET-1 expression levels are predictive of lung transplant outcomes and represent a valuable monitoring tool for surgeons; however, traditional techniques that measure [ET-1] are not suitable for the transplant setting. Herein, we demonstrate a new assay that rapidly measures ET-1 peptide levels in lung perfusate.

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Using the inherent chemistry of the endothelin-1 peptide to develop a rapid assay for pre-transplant donor lung assessment

Abstract

ASJC Scopus subject areas

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