Using white noise to gate organic transistors for dynamic monitoring of cultured cell layers

Jonathan Rivnay*, Pierre Leleux, Adel Hama, Marc Ramuz, Miriam Huerta, George G. Malliaras, Roisin M. Owens

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Impedance sensing of biological systems allows for monitoring of cell and tissue properties, including cell-substrate attachment, layer confluence, and the tightness' of an epithelial tissue. These properties are critical for electrical detection of tissue health and viability in applications such as toxicological screening. Organic transistors based on conducting polymers offer a promising route to efficiently transduce ionic currents to attain high quality impedance spectra, but collection of complete impedance spectra can be time consuming (minutes). By applying uniform white noise at the gate of an organic electrochemical transistor (OECT), and measuring the resulting current noise, we are able to dynamically monitor the impedance and thus integrity of cultured epithelial monolayers. We show that noise sourcing can be used to track rapid monolayer disruption due to compounds which interfere with dynamic polymerization events crucial for maintaining cytoskeletal integrity, and to resolve sub-second alterations to the monolayer integrity.

Original languageEnglish (US)
Article number11613
JournalScientific reports
Volume5
DOIs
StatePublished - Jun 26 2015

ASJC Scopus subject areas

  • General

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