@inbook{51b3a854aa394e248545c6f694fee65d,
title = "Using xenopus skin to study cilia development and function",
abstract = "Cilia are prevalent biological structures that are important for cell signaling and for generating fluid flow (or motility). Cilia are found throughout biology from single-celled organisms to vertebrates, and many model systems have been employed for their analysis. Here, we describe the use of Xenopus larval skin as a system for the study of ciliogenesis and ciliary function. In particular, we describe basic molecular and embryological manipulations and imaging techniques that have proven particularly useful for understanding the polarized beating of cilia and the generation of directed fluid flow (Werner & Mitchell, 2012). However, these same tools have the potential to benefit a large number of cilia-related biological questions.",
keywords = "Xenopus, cilia, ciliated epithelia, ciliogenesis, planar cell polarity",
author = "Werner, {Michael E.} and Mitchell, {Brian J.}",
note = "Funding Information: We would like to thank Jennifer Stubbs, Chris Kintner, and members of the Mitchell lab for helpful comments and discussions. M. E. W. is supported by postdoctoral fellowship from the American Heart Association, and B. J. M. is funded through the support of the NIH/NIGMS: R01GM089970.",
year = "2013",
doi = "10.1016/B978-0-12-397944-5.00010-9",
language = "English (US)",
isbn = "9780123979445",
series = "Methods in Enzymology",
publisher = "Academic Press Inc",
pages = "191--217",
booktitle = "Cilia, Part B",
}