USP17 is required for clathrin mediated endocytosis of epidermal growth factor receptor

Jakub Jaworski, Michelle de la Vega, Sarah J. Fletcher, Cheryl McFarlane, Michelle K. Greene, Andrew W. Smyth, Sandra V. Van Schaeybroeck, James A. Johnston, Christopher J. Scott, Joshua Z. Rappoport, James F. Burrows*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Previously we have shown that expression of the deubiquitinating enzyme USP17 is required for cell proliferation and motility. More recently we reported that USP17 deubiquitinates RCE1 isoform 2 and thus regulates the processing of 'CaaX' motif proteins. Here we now show that USP17 expression is induced by epidermal growth factor and that USP17 expression is required for clathrin mediated endocytosis of epidermal growth factor receptor. In addition, we show that USP17 is required for the endocytosis of transferrin, an archetypal substrate for clathrin mediated endocytosis, and that USP17 depletion impedes plasma membrane recruitment of the machinery required for clathrin mediated endocytosis. Thus, our data reveal that USP17 is necessary for epidermal growth factor receptor and transferrin endocytosis via clathrin coated pits, indicate this is mediated via the regulation of the recruitment of the components of the endocytosis machinery and suggest USP17 may play a general role in receptor endocytosis.

Original languageEnglish (US)
Pages (from-to)6964-6975
Number of pages12
JournalOncotarget
Volume5
Issue number16
DOIs
StatePublished - 2014

Keywords

  • Clathrin
  • Deubiquitinating
  • Endocytosis
  • Epidermal growth factor receptor
  • USP17

ASJC Scopus subject areas

  • Oncology

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