TY - JOUR
T1 - Utility of nine-color, 11-Parameter flow cytometry for detection of plasma cell neoplasms
T2 - A comparison with bone marrow morphologic findings and concurrent M-protein studies in serum and urine
AU - Behdad, Amir
AU - Ross, Charles W.
AU - Jacques, Joshua
AU - Kota, Usha
AU - Keren, David
AU - Stoolman, Lloyd
N1 - Publisher Copyright:
© American Society for Clinical Pathology.
PY - 2014/9/1
Y1 - 2014/9/1
N2 - Objectives: Multiparameter flow cytometry (MFC) is a widely available laboratory platform for the evaluation of plasma cell (PC) neoplasms. We assess the performance of a nine-color MFC assay that uses stain-lyse-fix processing of bone marrow aspirates, minimal wash steps, and high acquisition rates with analysis of up to 1.8 × 106cells.Methods: MFC results were compared with microscopic examinations, immunohistochemical studies, and serum/urine M-protein measurements from patients with documented or suspected PC neoplasms.Results: Sensitivity exceeded that of microscopic examinations, with or without immunohistochemistry. In patients with PC myeloma, clonal PC detection by MFC fell in concert with M-protein levels. However, in a subset of patients, MFC detected clonal PCs after serum/urine studies turned negative.Conclusions: The nine-color analytic cocktail eliminates duplication of PC gating reagents required for evaluation of the same epitopes using a five- or six-color approach. Fewer analytic cocktails result in lower instrument acquisition times per case, a significant factor for the large data sets required for optimal residual disease assessment. Finally, concurrent analysis of nine epitopes and two light scatter parameters aids detection of residual disease, particularly when it is mixed with polyclonal PCs.
AB - Objectives: Multiparameter flow cytometry (MFC) is a widely available laboratory platform for the evaluation of plasma cell (PC) neoplasms. We assess the performance of a nine-color MFC assay that uses stain-lyse-fix processing of bone marrow aspirates, minimal wash steps, and high acquisition rates with analysis of up to 1.8 × 106cells.Methods: MFC results were compared with microscopic examinations, immunohistochemical studies, and serum/urine M-protein measurements from patients with documented or suspected PC neoplasms.Results: Sensitivity exceeded that of microscopic examinations, with or without immunohistochemistry. In patients with PC myeloma, clonal PC detection by MFC fell in concert with M-protein levels. However, in a subset of patients, MFC detected clonal PCs after serum/urine studies turned negative.Conclusions: The nine-color analytic cocktail eliminates duplication of PC gating reagents required for evaluation of the same epitopes using a five- or six-color approach. Fewer analytic cocktails result in lower instrument acquisition times per case, a significant factor for the large data sets required for optimal residual disease assessment. Finally, concurrent analysis of nine epitopes and two light scatter parameters aids detection of residual disease, particularly when it is mixed with polyclonal PCs.
KW - Flow cytometry
KW - Minimal residual disease
KW - Multiple myeloma
KW - Plasma cell myeloma
KW - Residual disease
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U2 - 10.1309/AJCPO5GQPXF8QCEC
DO - 10.1309/AJCPO5GQPXF8QCEC
M3 - Article
C2 - 25125632
AN - SCOPUS:84907184135
SN - 0002-9173
VL - 142
SP - 398
EP - 410
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
IS - 3
ER -
