UV-induced changes in cell cycle and gene expression within rabbit lens epithelial cells

Duska Sidjanin, David Grdina, Gayle E. Woloschak*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Damage to lens epithelial cells is a probable initiation process in cataract formation mediated by UV radiation. In these experiments, we investigated the effects of exposure to 254 nm radiation on cell cycle progression and gene expression in the rabbit lens epithelial cell line N/N1003A. The RNA was harvested at various times following exposure to UV (254 nm) radiation and analyzed by dot-blot and northern blot hybridizations. These results revealed that during the first 6 h following exposure of the cells to UV, there was, associated with decreasing dose, a decrease in accumulation of transcripts specific for histones H3 and H4 and an increase in the mRNA encoding protein kinase C and β- and γ-actin. Using flow cytometry, we detected an accumulation of cells in G1/S phase of the cell cycle 1 h following exposure to 254 nm radiation. The observed changes in gene expression, especially the decreased accumulation of histone transcripts reported here, may play a role in UV-induced inhibition of cell cycle progression.

Original languageEnglish (US)
Pages (from-to)79-85
Number of pages7
JournalPhotochemistry and Photobiology
Volume63
Issue number1
DOIs
StatePublished - Jan 1996

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry

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