Vaccination with transforming growth factor-beta insensitive dendritic cells suppresses pulmonary metastases of renal carcinoma in mice

Feng Tian; Longxin Wang; Weijun Qin; Fuli Wang; Bin Song; Yu Li; Weihong Wen; Zheng Zhang; Kaichang Zhu; Qiang Zhang; Chung Lee; Weide Zhong; Yinglu Guo; He Wang

doi:10.1016/j.canlet.2008.06.025

Vaccination with transforming growth factor-beta insensitive dendritic cells suppresses pulmonary metastases of renal carcinoma in mice

Feng Tian, Longxin Wang, Weijun Qin, Fuli Wang, Bin Song, Yu Li, Weihong Wen, Zheng Zhang, Kaichang Zhu, Qiang Zhang, Chung Lee, Weide Zhong, Yinglu Guo^*, He Wang

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Dendritic cells (DCs) have been widely used as cancer vaccines. However, their functional abilities have often been suppressed by tumor-secreted immunosuppressants such as transforming growth factor-beta (TGF-β). We developed a new strategy using a TGF-β insensitive DC as cancer vaccine. The effect of this vaccine was tested in a murine pulmonary metastases model of renal carcinoma (Renca). Tumor lysate-pulsed DCs (TP-DCs) were infected with retrovirus containing gene of dominant negative TGF-β type II receptor (TβRIIDN) and thus made TGF-β insensitive. Vaccination of the mice bearing Renca pulmonary metastases with the TβRIIDN TP-DC induced powerful tumor-specific cytotoxic T lymphocyte (CTL) responses, suppressed pulmonary metastases, and prolonged survival times. These results suggest TGF-β-insensitive TP-DC vaccine can be used to enhance the antitumor efficacy of DC vaccine.

Original language	English (US)
Pages (from-to)	333-341
Number of pages	9
Journal	Cancer Letters
Volume	271
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2008.06.025
State	Published - Nov 28 2008

Keywords

Cancer immunotherapy
Dendritic cell
Renal carcinoma
Transforming growth factor-beta
Vaccination

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.canlet.2008.06.025

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title = "Vaccination with transforming growth factor-beta insensitive dendritic cells suppresses pulmonary metastases of renal carcinoma in mice",

abstract = "Dendritic cells (DCs) have been widely used as cancer vaccines. However, their functional abilities have often been suppressed by tumor-secreted immunosuppressants such as transforming growth factor-beta (TGF-β). We developed a new strategy using a TGF-β insensitive DC as cancer vaccine. The effect of this vaccine was tested in a murine pulmonary metastases model of renal carcinoma (Renca). Tumor lysate-pulsed DCs (TP-DCs) were infected with retrovirus containing gene of dominant negative TGF-β type II receptor (TβRIIDN) and thus made TGF-β insensitive. Vaccination of the mice bearing Renca pulmonary metastases with the TβRIIDN TP-DC induced powerful tumor-specific cytotoxic T lymphocyte (CTL) responses, suppressed pulmonary metastases, and prolonged survival times. These results suggest TGF-β-insensitive TP-DC vaccine can be used to enhance the antitumor efficacy of DC vaccine.",

keywords = "Cancer immunotherapy, Dendritic cell, Renal carcinoma, Transforming growth factor-beta, Vaccination",

author = "Feng Tian and Longxin Wang and Weijun Qin and Fuli Wang and Bin Song and Yu Li and Weihong Wen and Zheng Zhang and Kaichang Zhu and Qiang Zhang and Chung Lee and Weide Zhong and Yinglu Guo and He Wang",

note = "Funding Information: This study was supported by grants from the Nature Science Foundation of China (Project No. 30300413 and 30672097) and the Technology Plan Emphasis Item of Peking (Project No. D0206011000091 and 7072084).",

year = "2008",

month = nov,

day = "28",

doi = "10.1016/j.canlet.2008.06.025",

language = "English (US)",

volume = "271",

pages = "333--341",

journal = "Cancer Letters",

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TY - JOUR

T1 - Vaccination with transforming growth factor-beta insensitive dendritic cells suppresses pulmonary metastases of renal carcinoma in mice

AU - Tian, Feng

AU - Wang, Longxin

AU - Qin, Weijun

AU - Wang, Fuli

AU - Song, Bin

AU - Li, Yu

AU - Wen, Weihong

AU - Zhang, Zheng

AU - Zhu, Kaichang

AU - Zhang, Qiang

AU - Lee, Chung

AU - Zhong, Weide

AU - Guo, Yinglu

AU - Wang, He

N1 - Funding Information: This study was supported by grants from the Nature Science Foundation of China (Project No. 30300413 and 30672097) and the Technology Plan Emphasis Item of Peking (Project No. D0206011000091 and 7072084).

PY - 2008/11/28

Y1 - 2008/11/28

N2 - Dendritic cells (DCs) have been widely used as cancer vaccines. However, their functional abilities have often been suppressed by tumor-secreted immunosuppressants such as transforming growth factor-beta (TGF-β). We developed a new strategy using a TGF-β insensitive DC as cancer vaccine. The effect of this vaccine was tested in a murine pulmonary metastases model of renal carcinoma (Renca). Tumor lysate-pulsed DCs (TP-DCs) were infected with retrovirus containing gene of dominant negative TGF-β type II receptor (TβRIIDN) and thus made TGF-β insensitive. Vaccination of the mice bearing Renca pulmonary metastases with the TβRIIDN TP-DC induced powerful tumor-specific cytotoxic T lymphocyte (CTL) responses, suppressed pulmonary metastases, and prolonged survival times. These results suggest TGF-β-insensitive TP-DC vaccine can be used to enhance the antitumor efficacy of DC vaccine.

AB - Dendritic cells (DCs) have been widely used as cancer vaccines. However, their functional abilities have often been suppressed by tumor-secreted immunosuppressants such as transforming growth factor-beta (TGF-β). We developed a new strategy using a TGF-β insensitive DC as cancer vaccine. The effect of this vaccine was tested in a murine pulmonary metastases model of renal carcinoma (Renca). Tumor lysate-pulsed DCs (TP-DCs) were infected with retrovirus containing gene of dominant negative TGF-β type II receptor (TβRIIDN) and thus made TGF-β insensitive. Vaccination of the mice bearing Renca pulmonary metastases with the TβRIIDN TP-DC induced powerful tumor-specific cytotoxic T lymphocyte (CTL) responses, suppressed pulmonary metastases, and prolonged survival times. These results suggest TGF-β-insensitive TP-DC vaccine can be used to enhance the antitumor efficacy of DC vaccine.

KW - Cancer immunotherapy

KW - Dendritic cell

KW - Renal carcinoma

KW - Transforming growth factor-beta

KW - Vaccination

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Vaccination with transforming growth factor-beta insensitive dendritic cells suppresses pulmonary metastases of renal carcinoma in mice

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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