Vaccination with transforming growth factor-beta insensitive dendritic cells suppresses pulmonary metastases of renal carcinoma in mice

Feng Tian, Longxin Wang, Weijun Qin, Fuli Wang, Bin Song, Yu Li, Weihong Wen, Zheng Zhang, Kaichang Zhu, Qiang Zhang, Chung Lee, Weide Zhong, Yinglu Guo*, He Wang

*Corresponding author for this work

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Dendritic cells (DCs) have been widely used as cancer vaccines. However, their functional abilities have often been suppressed by tumor-secreted immunosuppressants such as transforming growth factor-beta (TGF-β). We developed a new strategy using a TGF-β insensitive DC as cancer vaccine. The effect of this vaccine was tested in a murine pulmonary metastases model of renal carcinoma (Renca). Tumor lysate-pulsed DCs (TP-DCs) were infected with retrovirus containing gene of dominant negative TGF-β type II receptor (TβRIIDN) and thus made TGF-β insensitive. Vaccination of the mice bearing Renca pulmonary metastases with the TβRIIDN TP-DC induced powerful tumor-specific cytotoxic T lymphocyte (CTL) responses, suppressed pulmonary metastases, and prolonged survival times. These results suggest TGF-β-insensitive TP-DC vaccine can be used to enhance the antitumor efficacy of DC vaccine.

Original languageEnglish (US)
Pages (from-to)333-341
Number of pages9
JournalCancer Letters
Volume271
Issue number2
DOIs
StatePublished - Nov 28 2008

Keywords

  • Cancer immunotherapy
  • Dendritic cell
  • Renal carcinoma
  • Transforming growth factor-beta
  • Vaccination

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Tian, F., Wang, L., Qin, W., Wang, F., Song, B., Li, Y., Wen, W., Zhang, Z., Zhu, K., Zhang, Q., Lee, C., Zhong, W., Guo, Y., & Wang, H. (2008). Vaccination with transforming growth factor-beta insensitive dendritic cells suppresses pulmonary metastases of renal carcinoma in mice. Cancer Letters, 271(2), 333-341. https://doi.org/10.1016/j.canlet.2008.06.025