Validation of chimerism in pediatric recipients of allogeneic hematopoietic stem cell transplantation (HSCT) a comparison between two methods: Real-time PCR (qPCR) vs. variable number tandem repeats PCR (VNTR PCR)

Morris Kletzel*, Wei Huang, Marie Olszewski, Sana Khan

*Corresponding author for this work

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Post-hematopoietic stem cell transplantation (HSCT) chimerism monitoring is important to assess relapse and therapeutic intervention. The purpose of our study is to compare two methods variable number tandem repeats (VNTR) vs. quantitative real- time polymerase chain reaction (qPCR) in terms of determining chimerism. 127 (peripheral blood n = 112, bone marrow n = 15) samples were simultaneously tested by VNTR using AP O-B, D1S80, D1S111, D17S30, gene loci SRY and ZP3 and qPCR using 34 assays (CA001-CA034) that are designed to a bi-allelic insertion/deletion (indel) polymorphism in the human genome. Samples were separated in three subsets: total WBC, T-cell and Myeloid cells. Extraction of DNA was performed then quantified. We analyzed column statistics, paired t-test and regression analysis for both methods. There was complete correlation between the two methods. The simplicity and rapidity of the test results from the qPCR method is more efficient and accurate to assess chimerism.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalChimerism
Volume4
Issue number1
DOIs
StatePublished - Jan 1 2013

Keywords

  • Chimerism
  • HSCT
  • PCR
  • QPCR
  • Real-time PCR
  • Variable number tandem repeats
  • VNTR

ASJC Scopus subject areas

  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Validation of chimerism in pediatric recipients of allogeneic hematopoietic stem cell transplantation (HSCT) a comparison between two methods: Real-time PCR (qPCR) vs. variable number tandem repeats PCR (VNTR PCR)'. Together they form a unique fingerprint.

  • Cite this