Variant chromosomal arrangement of adult β-globin genes in rat

Milena Stevanović; Tatjana Paunesku; Danica Radosavljević; Radoje Drmanac; Radomir Crkvenjakov

doi:10.1016/0378-1119(89)90099-1

Variant chromosomal arrangement of adult β-globin genes in rat

Milena Stevanović, Tatjana Paunesku, Danica Radosavljević, Radoje Drmanac, Radomir Crkvenjakov^*

^*Corresponding author for this work

Radiation Oncology

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

The genomic organization of three haplotypes of β-globin genes was determined to resolve the question of the number of those genes in rat. Haplotype a, found in inbred strain DA, has three genes or pseudogenes, while haplotypes b, found in AO, Y5 and Wistar strains, and c, found in Wistar strain, have five genes or pseudogenes each. In haplotypes b and c, the first gene is of β^major type and the remaining four are of β^minir type. Partial sequencing of six out of 13 genes shows that duplications of β^minor genes are causing polymorphism in a number of genes. Also, in haplotype b two β^minor genes have a 6.5-kb intron 2, while in haplotype c only one β^minor gene contains such a large intron 2. The three structurally different haplotypes described are not interconvertible by single recombination events. The results indicate that the rat has the highest number of adult β-globin genes found in mammals so far.

Original language	English (US)
Pages (from-to)	139-150
Number of pages	12
Journal	Gene
Volume	79
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(89)90099-1
State	Published - Jun 30 1989

Funding

This researchw as supportedin part by SIZ for Science,S erbiaa nd U.S.-YugoslavJ oint Board on Scientific and TechnologicalC ooperation,G rant JF 789.

Keywords

Recombinant DNA
Southern-blot analysis
genomic phage λ library
haplotypes differing in number of genes
restriction maps

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(89)90099-1

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title = "Variant chromosomal arrangement of adult β-globin genes in rat",

abstract = "The genomic organization of three haplotypes of β-globin genes was determined to resolve the question of the number of those genes in rat. Haplotype a, found in inbred strain DA, has three genes or pseudogenes, while haplotypes b, found in AO, Y5 and Wistar strains, and c, found in Wistar strain, have five genes or pseudogenes each. In haplotypes b and c, the first gene is of βmajor type and the remaining four are of βminir type. Partial sequencing of six out of 13 genes shows that duplications of βminor genes are causing polymorphism in a number of genes. Also, in haplotype b two βminor genes have a 6.5-kb intron 2, while in haplotype c only one βminor gene contains such a large intron 2. The three structurally different haplotypes described are not interconvertible by single recombination events. The results indicate that the rat has the highest number of adult β-globin genes found in mammals so far.",

keywords = "Recombinant DNA, Southern-blot analysis, genomic phage λ library, haplotypes differing in number of genes, restriction maps",

author = "Milena Stevanovi{\'c} and Tatjana Paunesku and Danica Radosavljevi{\'c} and Radoje Drmanac and Radomir Crkvenjakov",

note = "Funding Information: This researchw as supportedin part by SIZ for Science,S erbiaa nd U.S.-YugoslavJ oint Board on Scientific and TechnologicalC ooperation,G rant JF 789.",

year = "1989",

month = jun,

day = "30",

doi = "10.1016/0378-1119(89)90099-1",

language = "English (US)",

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T1 - Variant chromosomal arrangement of adult β-globin genes in rat

AU - Stevanović, Milena

AU - Paunesku, Tatjana

AU - Radosavljević, Danica

AU - Drmanac, Radoje

AU - Crkvenjakov, Radomir

N1 - Funding Information: This researchw as supportedin part by SIZ for Science,S erbiaa nd U.S.-YugoslavJ oint Board on Scientific and TechnologicalC ooperation,G rant JF 789.

PY - 1989/6/30

Y1 - 1989/6/30

N2 - The genomic organization of three haplotypes of β-globin genes was determined to resolve the question of the number of those genes in rat. Haplotype a, found in inbred strain DA, has three genes or pseudogenes, while haplotypes b, found in AO, Y5 and Wistar strains, and c, found in Wistar strain, have five genes or pseudogenes each. In haplotypes b and c, the first gene is of βmajor type and the remaining four are of βminir type. Partial sequencing of six out of 13 genes shows that duplications of βminor genes are causing polymorphism in a number of genes. Also, in haplotype b two βminor genes have a 6.5-kb intron 2, while in haplotype c only one βminor gene contains such a large intron 2. The three structurally different haplotypes described are not interconvertible by single recombination events. The results indicate that the rat has the highest number of adult β-globin genes found in mammals so far.

AB - The genomic organization of three haplotypes of β-globin genes was determined to resolve the question of the number of those genes in rat. Haplotype a, found in inbred strain DA, has three genes or pseudogenes, while haplotypes b, found in AO, Y5 and Wistar strains, and c, found in Wistar strain, have five genes or pseudogenes each. In haplotypes b and c, the first gene is of βmajor type and the remaining four are of βminir type. Partial sequencing of six out of 13 genes shows that duplications of βminor genes are causing polymorphism in a number of genes. Also, in haplotype b two βminor genes have a 6.5-kb intron 2, while in haplotype c only one βminor gene contains such a large intron 2. The three structurally different haplotypes described are not interconvertible by single recombination events. The results indicate that the rat has the highest number of adult β-globin genes found in mammals so far.

KW - Recombinant DNA

KW - Southern-blot analysis

KW - genomic phage λ library

KW - haplotypes differing in number of genes

KW - restriction maps

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Variant chromosomal arrangement of adult β-globin genes in rat

Abstract

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Keywords

ASJC Scopus subject areas

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