Variants of vasoactive intestinal peptide in mouse mast cells and rat basophilic leukemia cells

Barry K. Wershil, Christoph W. Turck, Sunil P. Sreedharan, Jinhong Yang, Songzu An, Stephen J. Galli, Edward J. Goetzl*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


Radioimmunoassays for neuroendocrine vasoactive intestinal peptide (VIP1-28) detected 30-120 fmol of structurally related peptides in extracts of 107 mouse peritoneal mast cells, bone marrow-derived mast cells, cultured PT-18 and C1.MC/C57.1 lines of mast cells, and rat basophilic leukemia (RBL) cells. No VIP was found in peritoneal cells of mast cell-deficient WBB6F1-W/Wv mice, whereas the amounts extracted from peritoneal cells of the congenic normal (WBB6F1-+/+) mice were similar to those from cultured mouse mast cells. Sephadex G-25 gel filtration resolved two different-sized variants of VIP from mouse mast cells and RBL cells. Amino acid sequence analyses showed that the smaller variant is VIP10-28. The principal amino-terminally larger variant of VIP from C1.MC/C57.1 mouse mast cells and RBL cells exhibited amino acid sequence homology with VIP(-6)-28, and this sequence was established for the corresponding larger VIP from PT-18 mast cells. Polymerase chain reaction amplification of two different substituent sequences of prepro VIP in RBL cell RNA identified the VIP message. VIP10-28 was released from mouse mast cells concurrently with histamine by IgE-dependent stimulation. Rodent mast cell-derived VIP thus consists of both the truncated VIP10-28 and amino-terminally larger forms that appear to be generated by peptidolysis of a prepro VIP similar to that found in neural cells.

Original languageEnglish (US)
Pages (from-to)369-378
Number of pages10
JournalCellular Immunology
Issue number2
StatePublished - Oct 15 1993

ASJC Scopus subject areas

  • Immunology


Dive into the research topics of 'Variants of vasoactive intestinal peptide in mouse mast cells and rat basophilic leukemia cells'. Together they form a unique fingerprint.

Cite this