VE-cadherin-cre-recombinase transgenic mouse: A tool for lineage analysis and gene deletion in endothelial cells

Jackelyn A. Alva, Ann C. Zovein, Arnaud Monvoisin, Thomas Murphy, Anthony Salazar, Natasha L. Harvey, Peter Carmeliet, M. Luisa Iruela-Arispe*

*Corresponding author for this work

Research output: Contribution to journalArticle

270 Scopus citations

Abstract

The ability to target gene deletion to a specific cellular compartment via the Cre/loxP system has been a powerful tool in the analysis of broadly expressed genes. Here, we report the generation of a transgenic mouse line in which expression of Cre-recombinase is under the regulatory control of the VE-Cadherin promoter. Temporal distribution and activity of the enzyme was evaluated with two independent Cre reporter lines. Histological analysis was performed throughout development and in the adult. Recombination of lox P sites with subsequent expression of β-galactosidase or GFP was detected as early as E7.5 in endothelial cells of the yolk sac. Progressive staining of the embryonic vasculature was noted from E8.5-13.5; however, more contiguous reporter expression was only seen by E14.5 onward in all endotlielial compartments including arteries, veins, and capillaries. In addition, we found Cre activity in lymphatic endothelial cells. Unlike other endothelial-specific Cre mice, this model showed expression in the adult quiescent vasculature. Furthermore, the constitutive nature of the VE-Cadherin promoter in the adult can be advantageous for analysis of gene deletion in pathological settings.

Original languageEnglish (US)
Pages (from-to)759-767
Number of pages9
JournalDevelopmental Dynamics
Volume235
Issue number3
DOIs
StatePublished - Mar 2006

Keywords

  • Blood vessel
  • Cadherin
  • Cardiovascular system
  • Hematopoietic progenitors
  • Vasculature

ASJC Scopus subject areas

  • Developmental Biology

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