Venom characterization of the Brazilian Pampa snake Bothrops pubescens by top-down and bottom-up proteomics

Darlene Lopes Rangel; Rafael D. Melani; Evelise Leis Carvalho; Juliano Tomazzoni Boldo; Tiago Gomes dos Santos; Neil L. Kelleher; Paulo Marcos Pinto

doi:10.1016/j.toxicon.2022.106937

Venom characterization of the Brazilian Pampa snake Bothrops pubescens by top-down and bottom-up proteomics

Darlene Lopes Rangel, Rafael D. Melani, Evelise Leis Carvalho, Juliano Tomazzoni Boldo, Tiago Gomes dos Santos, Neil L. Kelleher, Paulo Marcos Pinto^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The envenomation from the Bothrops genus is characterized by systemic and local effects caused by the main toxin families in the venom. In Bothrops pubescens venom we were able to identify 89 protein groups belonging to 13 toxin families with the bottom-up proteomics approach and 40 unique proteoforms belonging to 6 toxin families with the top-down proteomics approach. We also identified multi-proteoform complexes of dimeric L-amino acid oxidase using native top-down mass spectrometry.

Original language	English (US)
Article number	106937
Journal	Toxicon
Volume	220
DOIs	https://doi.org/10.1016/j.toxicon.2022.106937
State	Published - Dec 2022

Funding

This work was supported by the National Council for Scientific and Technological Development (CNPq-Brazil) , the Coordination for the Improvement of Higher Education Personnel (CAPES-Brazil) , the Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS-Brazil) , the National Institute of Health in a visiting scholar program supported by a grant from the National Institute of General Medical Sciences P41 GM108569 (N.L.K.), and the NIH Office of Director award S10 OD025194 . D.L.R. received a research fellowship from CAPES-Brazil, and E.L.C. received a research fellowship from FAPERGS-Brazil. This is for Alice.

Keywords

Bothrops
Envenomation
L-amino acid Oxidase
Venomics

ASJC Scopus subject areas

Toxicology

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10.1016/j.toxicon.2022.106937

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title = "Venom characterization of the Brazilian Pampa snake Bothrops pubescens by top-down and bottom-up proteomics",

abstract = "The envenomation from the Bothrops genus is characterized by systemic and local effects caused by the main toxin families in the venom. In Bothrops pubescens venom we were able to identify 89 protein groups belonging to 13 toxin families with the bottom-up proteomics approach and 40 unique proteoforms belonging to 6 toxin families with the top-down proteomics approach. We also identified multi-proteoform complexes of dimeric L-amino acid oxidase using native top-down mass spectrometry.",

keywords = "Bothrops, Envenomation, L-amino acid Oxidase, Venomics",

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language = "English (US)",

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AU - Rangel, Darlene Lopes

AU - Melani, Rafael D.

AU - Carvalho, Evelise Leis

AU - Boldo, Juliano Tomazzoni

AU - Gomes dos Santos, Tiago

AU - Kelleher, Neil L.

AU - Pinto, Paulo Marcos

PY - 2022/12

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N2 - The envenomation from the Bothrops genus is characterized by systemic and local effects caused by the main toxin families in the venom. In Bothrops pubescens venom we were able to identify 89 protein groups belonging to 13 toxin families with the bottom-up proteomics approach and 40 unique proteoforms belonging to 6 toxin families with the top-down proteomics approach. We also identified multi-proteoform complexes of dimeric L-amino acid oxidase using native top-down mass spectrometry.

AB - The envenomation from the Bothrops genus is characterized by systemic and local effects caused by the main toxin families in the venom. In Bothrops pubescens venom we were able to identify 89 protein groups belonging to 13 toxin families with the bottom-up proteomics approach and 40 unique proteoforms belonging to 6 toxin families with the top-down proteomics approach. We also identified multi-proteoform complexes of dimeric L-amino acid oxidase using native top-down mass spectrometry.

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KW - Envenomation

KW - L-amino acid Oxidase

KW - Venomics

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Venom characterization of the Brazilian Pampa snake Bothrops pubescens by top-down and bottom-up proteomics

Abstract

Funding

Keywords

ASJC Scopus subject areas

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