Venom characterization of the Brazilian Pampa snake Bothrops pubescens by top-down and bottom-up proteomics

Darlene Lopes Rangel, Rafael D. Melani, Evelise Leis Carvalho, Juliano Tomazzoni Boldo, Tiago Gomes dos Santos, Neil L. Kelleher, Paulo Marcos Pinto*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The envenomation from the Bothrops genus is characterized by systemic and local effects caused by the main toxin families in the venom. In Bothrops pubescens venom we were able to identify 89 protein groups belonging to 13 toxin families with the bottom-up proteomics approach and 40 unique proteoforms belonging to 6 toxin families with the top-down proteomics approach. We also identified multi-proteoform complexes of dimeric L-amino acid oxidase using native top-down mass spectrometry.

Original languageEnglish (US)
Article number106937
JournalToxicon
Volume220
DOIs
StatePublished - Dec 2022

Keywords

  • Bothrops
  • Envenomation
  • L-amino acid Oxidase
  • Venomics

ASJC Scopus subject areas

  • Toxicology

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