Vertebrate slit, a secreted ligand for the transmembrane protein roundabout, is a repellent for olfactory bulb axons

Hua shun Li; Jin hui Chen; Wei Wu; Tanya Fagaly; Lijuan Zhou; Wenlin Yuan; Sophie Dupuis; Zhi hong Jiang; William Nash; Carrie Gick; David M. Ornitz; Jane Y. Wu; Yi Rao

doi:10.1016/S0092-8674(00)80591-7

Vertebrate slit, a secreted ligand for the transmembrane protein roundabout, is a repellent for olfactory bulb axons

Hua shun Li, Jin hui Chen, Wei Wu, Tanya Fagaly, Lijuan Zhou, Wenlin Yuan, Sophie Dupuis, Zhi hong Jiang, William Nash, Carrie Gick, David M. Ornitz, Jane Y. Wu^*, Yi Rao

^*Corresponding author for this work

Neurology

Research output: Contribution to journal › Article › peer-review

411 Scopus citations

Abstract

The olfactory bulb plays a central role in olfactory information processing through its connections with both peripheral and cortical structures. Axons projecting from the olfactory bulb to the telencephalon are guided by a repulsive activity in the septum. The molecular nature of the repellent is not known. We report here the isolation of vertebrate homologs of the Drosophila slit gene and show that Slit protein binds to the transmembrane protein Roundabout (Robo). Slit is expressed in the septum whereas Robo is expressed in the olfactory bulb. Functionally, Slit acts as a chemorepellent for olfactory bulb axons. These results establish a ligand- receptor relationship between two molecules important for neural development, suggest a role for Slit in olfactory bulb axon guidance, and reveal the existence of a new family of axon guidance molecules.

Original language	English (US)
Pages (from-to)	807-818
Number of pages	12
Journal	Cell
Volume	96
Issue number	6
DOIs	https://doi.org/10.1016/S0092-8674(00)80591-7
State	Published - Mar 19 1999

Funding

We are grateful to J. Price for helpful discussions; to P. Klein for comments on the manuscript; to M. Tessier-Lavigne for the stable Netrin-expressing cell line; to D. Fisher for a Sema III cDNA; to M. Tessier-Lavigne and C. Goodman for communication prior to publication; to NIH, NSF, NNSFC, and SCST for support; to the John Merck Fund, NNSFC, and the Leukemia Society of America for scholar awards (to Y. R. and J. Y. W.).

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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title = "Vertebrate slit, a secreted ligand for the transmembrane protein roundabout, is a repellent for olfactory bulb axons",

abstract = "The olfactory bulb plays a central role in olfactory information processing through its connections with both peripheral and cortical structures. Axons projecting from the olfactory bulb to the telencephalon are guided by a repulsive activity in the septum. The molecular nature of the repellent is not known. We report here the isolation of vertebrate homologs of the Drosophila slit gene and show that Slit protein binds to the transmembrane protein Roundabout (Robo). Slit is expressed in the septum whereas Robo is expressed in the olfactory bulb. Functionally, Slit acts as a chemorepellent for olfactory bulb axons. These results establish a ligand- receptor relationship between two molecules important for neural development, suggest a role for Slit in olfactory bulb axon guidance, and reveal the existence of a new family of axon guidance molecules.",

author = "Li, {Hua shun} and Chen, {Jin hui} and Wei Wu and Tanya Fagaly and Lijuan Zhou and Wenlin Yuan and Sophie Dupuis and Jiang, {Zhi hong} and William Nash and Carrie Gick and Ornitz, {David M.} and Wu, {Jane Y.} and Yi Rao",

note = "Funding Information: We are grateful to J. Price for helpful discussions; to P. Klein for comments on the manuscript; to M. Tessier-Lavigne for the stable Netrin-expressing cell line; to D. Fisher for a Sema III cDNA; to M. Tessier-Lavigne and C. Goodman for communication prior to publication; to NIH, NSF, NNSFC, and SCST for support; to the John Merck Fund, NNSFC, and the Leukemia Society of America for scholar awards (to Y. R. and J. Y. W.).",

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T1 - Vertebrate slit, a secreted ligand for the transmembrane protein roundabout, is a repellent for olfactory bulb axons

AU - Li, Hua shun

AU - Chen, Jin hui

AU - Wu, Wei

AU - Fagaly, Tanya

AU - Zhou, Lijuan

AU - Yuan, Wenlin

AU - Dupuis, Sophie

AU - Jiang, Zhi hong

AU - Nash, William

AU - Gick, Carrie

AU - Ornitz, David M.

AU - Wu, Jane Y.

AU - Rao, Yi

N1 - Funding Information: We are grateful to J. Price for helpful discussions; to P. Klein for comments on the manuscript; to M. Tessier-Lavigne for the stable Netrin-expressing cell line; to D. Fisher for a Sema III cDNA; to M. Tessier-Lavigne and C. Goodman for communication prior to publication; to NIH, NSF, NNSFC, and SCST for support; to the John Merck Fund, NNSFC, and the Leukemia Society of America for scholar awards (to Y. R. and J. Y. W.).

PY - 1999/3/19

Y1 - 1999/3/19

N2 - The olfactory bulb plays a central role in olfactory information processing through its connections with both peripheral and cortical structures. Axons projecting from the olfactory bulb to the telencephalon are guided by a repulsive activity in the septum. The molecular nature of the repellent is not known. We report here the isolation of vertebrate homologs of the Drosophila slit gene and show that Slit protein binds to the transmembrane protein Roundabout (Robo). Slit is expressed in the septum whereas Robo is expressed in the olfactory bulb. Functionally, Slit acts as a chemorepellent for olfactory bulb axons. These results establish a ligand- receptor relationship between two molecules important for neural development, suggest a role for Slit in olfactory bulb axon guidance, and reveal the existence of a new family of axon guidance molecules.

AB - The olfactory bulb plays a central role in olfactory information processing through its connections with both peripheral and cortical structures. Axons projecting from the olfactory bulb to the telencephalon are guided by a repulsive activity in the septum. The molecular nature of the repellent is not known. We report here the isolation of vertebrate homologs of the Drosophila slit gene and show that Slit protein binds to the transmembrane protein Roundabout (Robo). Slit is expressed in the septum whereas Robo is expressed in the olfactory bulb. Functionally, Slit acts as a chemorepellent for olfactory bulb axons. These results establish a ligand- receptor relationship between two molecules important for neural development, suggest a role for Slit in olfactory bulb axon guidance, and reveal the existence of a new family of axon guidance molecules.

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Vertebrate slit, a secreted ligand for the transmembrane protein roundabout, is a repellent for olfactory bulb axons

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