Vertical column electrophoresis in sephadex G-200

Thomas A. Tedesco; Robert Bonow; William J. Mellman

doi:10.1016/0003-2697(72)90409-5

Vertical column electrophoresis in sephadex G-200

Thomas A. Tedesco^*, Robert Bonow, William J. Mellman

^*Corresponding author for this work

Medicine, Cardiology Division

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

A method for electrophoretic separation in a vertical column packed with Sephadex G-200 superfine media is described. Collection of electrophoretically separated components is accomplished by converting the column to an eluting system after electrophoresis. The method has been used successfully to isolate human galactose-1-phosphate uridyltransferase. The application of a human serum sample was used to demonstrate the fractionation potential of the method.

Original language	English (US)
Pages (from-to)	173-180
Number of pages	8
Journal	Analytical Biochemistry
Volume	46
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(72)90409-5
State	Published - Jan 1 1972

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

Access to Document

10.1016/0003-2697(72)90409-5

Fingerprint Dive into the research topics of 'Vertical column electrophoresis in sephadex G-200'. Together they form a unique fingerprint.

Cite this

@article{1ddc3bebb28340dfa15fad6159c3fe2e,

title = "Vertical column electrophoresis in sephadex G-200",

abstract = "A method for electrophoretic separation in a vertical column packed with Sephadex G-200 superfine media is described. Collection of electrophoretically separated components is accomplished by converting the column to an eluting system after electrophoresis. The method has been used successfully to isolate human galactose-1-phosphate uridyltransferase. The application of a human serum sample was used to demonstrate the fractionation potential of the method.",

author = "Tedesco, {Thomas A.} and Robert Bonow and Mellman, {William J.}",

year = "1972",

month = jan,

day = "1",

doi = "10.1016/0003-2697(72)90409-5",

language = "English (US)",

volume = "46",

pages = "173--180",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Vertical column electrophoresis in sephadex G-200

AU - Tedesco, Thomas A.

AU - Bonow, Robert

AU - Mellman, William J.

PY - 1972/1/1

Y1 - 1972/1/1

N2 - A method for electrophoretic separation in a vertical column packed with Sephadex G-200 superfine media is described. Collection of electrophoretically separated components is accomplished by converting the column to an eluting system after electrophoresis. The method has been used successfully to isolate human galactose-1-phosphate uridyltransferase. The application of a human serum sample was used to demonstrate the fractionation potential of the method.

AB - A method for electrophoretic separation in a vertical column packed with Sephadex G-200 superfine media is described. Collection of electrophoretically separated components is accomplished by converting the column to an eluting system after electrophoresis. The method has been used successfully to isolate human galactose-1-phosphate uridyltransferase. The application of a human serum sample was used to demonstrate the fractionation potential of the method.

UR - http://www.scopus.com/inward/record.url?scp=0015304390&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015304390&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(72)90409-5

DO - 10.1016/0003-2697(72)90409-5

M3 - Article

C2 - 5017655

AN - SCOPUS:0015304390

VL - 46

SP - 173

EP - 180

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 1

ER -