Vertical column electrophoresis in sephadex G-200

Thomas A. Tedesco*, Robert Bonow, William J. Mellman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

A method for electrophoretic separation in a vertical column packed with Sephadex G-200 superfine media is described. Collection of electrophoretically separated components is accomplished by converting the column to an eluting system after electrophoresis. The method has been used successfully to isolate human galactose-1-phosphate uridyltransferase. The application of a human serum sample was used to demonstrate the fractionation potential of the method.

Original languageEnglish (US)
Pages (from-to)173-180
Number of pages8
JournalAnalytical Biochemistry
Volume46
Issue number1
DOIs
StatePublished - Jan 1 1972

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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